UniProt Accession

 DYR_MYCPN; P78028 

Genbank Protein ID

 U00089 

Genbank Nucleotide ID

 AAB96163.1 

Protein Name

 Dihydrofolate reductase 

Protein Synonyms/Alias

  

Gene Name

 folA 

Gene Synonyms/Alias

 dhfR; MPN_321; MP515 

Created Date

 July 27, 2013 

Organism

 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 

NCBI Taxa ID

 272634 

Lysine Modification

Position	Peptide	Type	References
25	NSLPWRIKAELQHFR	acetylation	[1]
133	YTGDKYLKVDFSPFE	acetylation	[1]
143	FSPFELTKEISFAEF	acetylation	[1]
157	FKVAYYHKIAR****	acetylation	[1]

Reference

 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 

Functional Description

 Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity). 

Sequence Annotation

 DOMAIN 1 160 DHFR.
 NP_BIND 6 7 NADP (By similarity).
 NP_BIND 14 19 NADP (By similarity).
 NP_BIND 43 46 NADP (By similarity).
 NP_BIND 62 65 NADP (By similarity).
 NP_BIND 101 106 NADP (By similarity).
 REGION 5 7 Substrate binding (By similarity).
 BINDING 27 27 Substrate (By similarity).
 BINDING 32 32 Substrate (By similarity).
 BINDING 57 57 Substrate (By similarity).
 BINDING 120 120 Substrate (By similarity).  

Keyword

 Complete proteome; NADP; One-carbon metabolism; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 160 AA 

Protein Sequence

Gene Ontology

 GO:0004146; F:dihydrofolate reductase activity; IEA:EC.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR012259; DHFR.
 IPR024072; DHFR-like_dom.
 IPR017925; DHFR_CS.
 IPR001796; DHFR_dom. 

Pfam

 PF00186; DHFR_1 

SMART

  

PROSITE

 PS00075; DHFR_1
 PS51330; DHFR_2 

PRINTS

 PR00070; DHFR.