UniProt Accession

 MD2L1_HUMAN; Q13257; Q53F56; Q548X9; Q6IRW7; Q8IZX3 

Genbank Protein ID

 U65410; AF202273; AF202269; AF202270; AF202271; AF202272; U31278; AJ000186; AB056160; AF394735; AK298228; AK313827; AK223433; AC097173; CH471056; CH471056; BC000356; BC005945; BC070283 

Genbank Nucleotide ID

 AAC50781.1; AAK38174.1; AAK38174.1; AAK38174.1; AAK38174.1; AAK38174.1; AAC52060.1; CAA03943.1; BAB63410.1; AAN74648.1; BAG60497.1; BAG36562.1; BAD97153.1; AAY40945.1; EAX05271.1; EAX05273.1; AAH00356.1; AAH05945.1; AAH70283.1 

Protein Name

 Mitotic spindle assembly checkpoint protein MAD2A 

Protein Synonyms/Alias

 HsMAD2; Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1 

Gene Name

 MAD2L1 

Gene Synonyms/Alias

 MAD2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
108	QFDIECDKTAKDDSA	acetylation	[1]
108	QFDIECDKTAKDDSA	ubiquitination	[1, 2]
111	IECDKTAKDDSAPRE	ubiquitination	[1]
122	APREKSQKAIQDEIR	ubiquitination	[1, 2, 3, 4, 5]
166	KDLVVPEKWEESGPQ	ubiquitination	[3, 6, 7, 8]
192	SFTTTIHKVNSMVAY	ubiquitination	[2, 6, 8, 9]
200	VNSMVAYKIPVND**	ubiquitination	[1, 2, 3, 4, 5, 10]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. 

Sequence Annotation

 DOMAIN 14 197 HORMA.
 REGION 195 205 Required for assuming the closed
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 195 195 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 205 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0072686; C:mitotic spindle; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000070; P:mitotic sister chromatid segregation; IEA:Compara.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0060564; P:negative regulation of mitotic anaphase-promoting complex activity; IDA:UniProtKB.
 GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; IMP:UniProtKB. 

Interpro

 IPR003511; HORMA_DNA-bd. 

Pfam

 PF02301; HORMA 

SMART

  

PROSITE

 PS50815; HORMA 

PRINTS