CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013467
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 FAST kinase domain-containing protein 1 
Protein Synonyms/Alias
  
Gene Name
 FASTKD1 
Gene Synonyms/Alias
 KIAA1800 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
322PIAGPEEKKQLKSTMubiquitination[1]
323IAGPEEKKQLKSTMLubiquitination[1]
360SRNSCLIKRVTSVLHacetylation[2, 3]
482KQLKLLQKLDHYGRQacetylation[4]
482KQLKLLQKLDHYGRQubiquitination[1]
691RFCQQYNKGIGGMDGubiquitination[1]
728VLTPYYHKVDFECILubiquitination[1]
737DFECILDKRKKPLPYubiquitination[1]
785ALEFLDSKALCRNIPubiquitination[1, 3, 5]
795CRNIPHMKGKSAMKKubiquitination[6]
797NIPHMKGKSAMKKRHubiquitination[6]
801MKGKSAMKKRHLEILubiquitination[6]
844ECIFGEVKSCL****ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 DOMAIN 777 837 RAP.
 MOD_RES 360 360 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 847 AA 
Protein Sequence
MKKTPVFLES LVTNMLRLRA ICPFSWRVFQ FRPISCEPLI IQMNKCTDEE QMFGFIERNK 60
AILSEKQVGC AFDMLWKLQK QKTSLLKNAE YVRDHPQFLT LHNLATNKFK LMNDDTLVNV 120
LYVTQQFAGE AHDPLVEALV TEAWRRLERF DIKLLSEFSS CLADQHLYFS PLMGKIADIV 180
HRNLETTQDL SSLSVLMVNI SSLISRHFQQ QLVNKTELLF DTIDSSEVNV AKSIAKFLRN 240
VRYRYQPLLE RCNNVFLSNV DHLDLDSISK ILSVYKFLQF NSFEFIIMAK KKLTEMIPLC 300
NHPASFVKLF VALGPIAGPE EKKQLKSTML LMSEDLTGEQ ALAVLGAMGD MESRNSCLIK 360
RVTSVLHKHL DGYKPLELLK ITQELTFLHF QRKEFFAKLR ELLLSYLKNS FIPTEVSVLV 420
RAISLLPSPH LDEVGISRIE AVLPQCDLNN LSSFATSVLR WIQHDHMYLD NMTAKQLKLL 480
QKLDHYGRQR LQHSNSLDLL RKELKSLKGN TFPESLLEEM IATLQHFMDD INYINVGEIA 540
SFISSTDYLS TLLLDRIASV AVQQIEKIHP FTIPAIIRPF SVLNYDPPQR DEFLGTCVQH 600
LNSYLGILDP FILVFLGFSL ATLEYFPEDL LKAIFNIKFL ARLDSQLEIL SPSRSARVQF 660
HLMELNRSVC LECPEFQIPW FHDRFCQQYN KGIGGMDGTQ QQIFKMLAEV LGGINCVKAS 720
VLTPYYHKVD FECILDKRKK PLPYGSHNIA LGQLPEMPWE SNIEIVGSRL PPGAERIALE 780
FLDSKALCRN IPHMKGKSAM KKRHLEILGY RVIQISQFEW NSMALSTKDA RMDYLRECIF 840
GEVKSCL 847 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0004672; F:protein kinase activity; IEA:InterPro.
 GO:0045333; P:cellular respiration; ISS:UniProtKB.
 GO:0006468; P:protein phosphorylation; IEA:GOC. 
Interpro
 IPR013579; FAST_2.
 IPR010622; FAST_Leu-rich.
 IPR013584; RAP. 
Pfam
 PF06743; FAST_1
 PF08368; FAST_2
 PF08373; RAP 
SMART
 SM00952; RAP 
PROSITE
 PS51286; RAP 
PRINTS