CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014018
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DDB1- and CUL4-associated factor 8 
Protein Synonyms/Alias
 WD repeat-containing protein 42A 
Gene Name
 DCAF8 
Gene Synonyms/Alias
 H326; WDR42A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
236LDFESGHKSNVFQAKubiquitination[1, 2]
374NENNGVLKKFCPHHLubiquitination[2]
438HRNNATVKGVNFYGPubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. 
Sequence Annotation
 REPEAT 191 230 WD 1.
 REPEAT 234 275 WD 2.
 REPEAT 281 321 WD 3.
 REPEAT 329 369 WD 4.
 REPEAT 385 424 WD 5.
 REPEAT 432 472 WD 6.
 REPEAT 476 515 WD 7.
 MOD_RES 99 99 Phosphoserine.
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 130 130 Phosphoserine.
 MOD_RES 204 204 Omega-N-methylarginine; by PRMT1.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Methylation; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MSSKGSSTDG RTDLANGSLS SSPEEMSGAE EGRETSSGIE VEASDLSLSL TGDDGGPNRT 60
STESRGTDTE SSGEDKDSDS MEDTGHYSIN DENRVHDRSE EEEEEEEEEE EEQPRRRVQR 120
KRANRDQDSS DDERALEDWV SSETSALPRP RWQALPALRE RELGSSARFV YEACGARVFV 180
QRFRLQHGLE GHTGCVNTLH FNQRGTWLAS GSDDLKVVVW DWVRRQPVLD FESGHKSNVF 240
QAKFLPNSGD STLAMCARDG QVRVAELSAT QCCKNTKRVA QHKGASHKLA LEPDSPCTFL 300
SAGEDAVVFT IDLRQDRPAS KLVVTKEKEK KVGLYTIYVN PANTHQFAVG GRDQFVRIYD 360
QRKIDENENN GVLKKFCPHH LVNSESKANI TCLVYSHDGT ELLASYNDED IYLFNSSHSD 420
GAQYVKRYKG HRNNATVKGV NFYGPKSEFV VSGSDCGHIF LWEKSSCQII QFMEGDKGGV 480
VNCLEPHPHL PVLATSGLDH DVKIWAPTAE ASTELTGLKD VIKKNKRERD EDSLHQTDLF 540
DSHMLWFLMH HLRQRRHHRR WREPGVGATD ADSDESPSSS DTSDEEEGPD RVQCMPS 597 
Gene Ontology
 GO:0080008; C:Cul4-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IC:UniProtKB. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS