CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031041
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA-binding motif, single-stranded-interacting protein 1 
Protein Synonyms/Alias
 cDNA FLJ61322, highly similar to RNA-binding motif,single-stranded-interacting protein 1 
Gene Name
 RBMS1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
101ILDKTTNKCKGYGFVubiquitination[1]
118DSPAAAQKAVSALKAubiquitination[1]
124QKAVSALKASGVQAQubiquitination[2]
207HFNGKFIKTPPGVSAubiquitination[1, 3, 4]
236KKRQNPNKYIPNGRPubiquitination[3, 5, 6]
300VSAYQVAKETRENKYubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 419 AA 
Protein Sequence
MGKVWKQQMY PQYATYYYPQ YLQAKQSLVP AHPMAPPSPS TTSSNNNSSS SSNSGWDQLS 60
KTNLYIRGLP PHTTDQDLVK LCQPYGKIVS TKAILDKTTN KCKGYGFVDF DSPAAAQKAV 120
SALKASGVQA QMAKQQEQDP TNLYISNLPL SMDEQELENM LKPFGQVIST RILRDSSGTS 180
RGVGFARMES TEKCEAVIGH FNGKFIKTPP GVSAPTEPLL CKFADGGQKK RQNPNKYIPN 240
GRPWHREGEA GMTLTYDPTT AAIQNGFYPS PYSIATNRMI TQTSITPYIA SPVSAYQVAK 300
ETRENKYRGS AIKVQSPSWM QPQPYILQHP GAVLTPSMEH TMSLQPASMI SPLAQQMSHL 360
SLGSTGTYMP ATSAMQGAYL PQYAHMQTTA VPVEEASGQQ QVAVETSNDH SPYTFQPNK 419 
Gene Ontology
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR002343; Hud_Sxl_RNA.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS
 PR00961; HUDSXLRNA.