CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016187
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable RNA-binding protein 23 
Protein Synonyms/Alias
 RNA-binding motif protein 23; RNA-binding region-containing protein 4; Splicing factor SF2 
Gene Name
 RBM23 
Gene Synonyms/Alias
 RNPC4; PP239 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
191DFFSAVGKVRDVRIIubiquitination[1, 2, 3, 4, 5]
245VQASQAEKNRLAAMAubiquitination[4]
257AMANNLQKGNGGPMRubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Probable RNA-binding protein. May be involved in pre- mRNA splicing process. 
Sequence Annotation
 DOMAIN 166 243 RRM 1.
 DOMAIN 263 341 RRM 2.
 MOD_RES 149 149 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MASDDFDIVI EAMLEAPYKK EEDEQQRKEV KKDYPSNTTS STSNSGNETS GSSTIGETSK 60
KKRSRSHNKS RDRKRSRSRD RDRYRRRNSR SRSPGRQCRH RSRSWDRRHG SESRSRDHRR 120
EDRVHYRSPP LATGYRYGHS KSPHFREKSP VREPVDNLSP EERDARTVFC MQLAARIRPR 180
DLEDFFSAVG KVRDVRIISD RNSRRSKGIA YVEFCEIQSV PLAIGLTGQR LLGVPIIVQA 240
SQAEKNRLAA MANNLQKGNG GPMRLYVGSL HFNITEDMLR GIFEPFGKID NIVLMKDSDT 300
GRSKGYGFIT FSDSECARRA LEQLNGFELA GRPMRVGHVT ERLDGGTDIT FPDGDQELDL 360
GSAGGRFQLM AKLAEGAGIQ LPSTAAAAAA AAAQAAALQL NGAVPLGALN PAALTALSPA 420
LNLASQCFQL SSLFTPQTM 439 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:InterPro. 
Interpro
 IPR006509; CC1_SF.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS