CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020346
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S phase cyclin A-associated protein in the endoplasmic reticulum 
Protein Synonyms/Alias
 S phase cyclin A-associated protein in the ER; Zinc finger protein 291 
Gene Name
 SCAPER 
Gene Synonyms/Alias
 KIAA1454; ZNF291; MSTP063 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
694RVEELLMKRKEQEARubiquitination[1]
744EAMEELQKKIQLKHDubiquitination[1]
745AMEELQKKIQLKHDEubiquitination[1]
749LQKKIQLKHDESIRRubiquitination[1]
784ANTDYAPKLTPYERKubiquitination[1]
928SGSWANNKVSALDRTubiquitination[1]
1088PTQEMKNKPSQGDPFubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 CCNA2/CDK2 regulatory protein that transiently maintains CCNA2 in the cytoplasm. 
Sequence Annotation
 ZN_FING 792 816 C2H2-type.  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1400 AA 
Protein Sequence
MMASFQRSNS HDKVRRIVAE EGRTARNLIA WSVPLESKDD DGKPKCQTGG KSKRTIQGTH 60
KTTKQSTAVD CKITSSTTGD KHFDKSPTKT RHPRKIDLRA RYWAFLFDNL RRAVDEIYVT 120
CESDQSVVEC KEVLMMLDNY VRDFKALIDW IQLQEKLEKT DAQSRPTSLA WEVKKMSPGR 180
HVIPSPSTDR INVTSNARRS LNFGGSTGTV PAPRLAPTGV SWADKVKAHH TGSTASSEIT 240
PAQSCPPMTV QKASRKNERK DAEGWETVQR GRPIRSRSTA VMPKVSLATE ATRSKDDSDK 300
ENVCLLPDES IQKGQFVGDG TSNTIESHPK DSLHSCDHPL AEKTQFTVST LDDVKNSGSI 360
RDNYVRTSEI SAVHIDTECV SVMLQAGTPP LQVNEEKFPA EKARIENEMD PSDISNSMAE 420
VLAKKEELAD RLEKANEEAI ASAIAEEEQL TREIEAEENN DINIETDNDS DFSASMGSGS 480
VSFCGMSMDW NDVLADYEAR ESWRQNTSWG DIVEEEPARP PGHGIHMHEK LSSPSRKRTI 540
AESKKKHEEK QMKAQQLREK LREEKTLKLQ KLLEREKDVR KWKEELLDQR RRMMEEKLLH 600
AEFKREVQLQ AIVKKAQEEE AKVNEIAFIN TLEAQNKRHD VLSKLKEYEQ RLNELQEERQ 660
RRQEEKQARD EAVQERKRAL EAERQARVEE LLMKRKEQEA RIEQQRQEKE KAREDAARER 720
ARDREERLAA LTAAQQEAME ELQKKIQLKH DESIRRHMEQ IEQRKEKAAE LSSGRHANTD 780
YAPKLTPYER KKQCSLCNVL ISSEVYLFSH VKGRKHQQAV RENTSIQGRE LSDEEVEHLS 840
LKKYIIDIVV ESTAPAEALK DGEERQKNKK KAKKIKARMN FRAKEYESLM ETKNSGSDSP 900
YKAKLQRLAK DLLKQVQVQD SGSWANNKVS ALDRTLGEIT RILEKENVAD QIAFQAAGGL 960
TALEHILQAV VPATNVNTVL RIPPKSLCNA INVYNLTCNN CSENCSDVLF SNKITFLMDL 1020
LIHQLTVYVP DENNTILGRN TNKQVFEGLT TGLLKVSAVV LGCLIANRPD GNCQPATPKI 1080
PTQEMKNKPS QGDPFNNRVQ DLISYVVNMG LIDKLCACFL SVQGPVDENP KMAIFLQHAA 1140
GLLHAMCTLC FAVTGRSYSI FDNNRQDPTG LTAALQATDL AGVLHMLYCV LFHGTILDPS 1200
TASPKENYTQ NTIQVAIQSL RFFNSFAALH LPAFQSIVGA EGLSLAFRHM ASSLLGHCSQ 1260
VSCESLLHEV IVCVGYFTVN HPDNQVIVQS GRHPTVLQKL CQLPFQYFSD PRLIKVLFPS 1320
LIAACYNNHQ NKIILEQEMS CVLLATFIQD LAQTPGQAEN QPYQPKGKCL GSQDYLELAN 1380
RFPQQAWEEA RQFFLKKEKK 1400 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. 
Interpro
 IPR015880; Znf_C2H2-like. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS