UniProt Accession

 GLUQ_ECOLI; P27305; P75662 

Genbank Protein ID

 U00096; AP009048; X64595; M34945 

Genbank Nucleotide ID

 AAC73255.3; BAB96721.2; AAA23686.1 

Protein Name

 Glutamyl-Q tRNA(Asp) synthetase 

Protein Synonyms/Alias

 Glu-Q-RSs 

Gene Name

 gluQ 

Gene Synonyms/Alias

 yadB; b0144; JW5892 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
165	GIIHADEKLAREDFI	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in position 34 of the tRNA(Asp), the wobble position of the QUC anticodon. Does not transfer glutamate to either tRNA(Glu) or tRNA(Gln). The incapacity of the glutamylated tRNA(Asp) to bind elongation factor Tu suggests that it is not involved in ribosomal protein biosynthesis. 

Sequence Annotation

 REGION 19 23 Glutamate binding.
 MOTIF 22 32 "HIGH" region.
 MOTIF 238 242 "KMSKS" region.
 METAL 111 111 Zinc.
 METAL 113 113 Zinc.
 METAL 125 125 Zinc.
 METAL 129 129 Zinc.
 BINDING 55 55 Glutamate.
 BINDING 182 182 Glutamate.
 BINDING 200 200 Glutamate.
 BINDING 241 241 ATP (By similarity).  

Keyword

 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Metal-binding; Nucleotide-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 308 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0004812; F:aminoacyl-tRNA ligase activity; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IDA:EcoCyc.
 GO:0008270; F:zinc ion binding; ISS:EcoCyc.
 GO:0043039; P:tRNA aminoacylation; IEA:InterPro.
 GO:0002097; P:tRNA wobble base modification; IDA:EcoCyc. 

Interpro

 IPR022380; Glu-Q_TRNA(Asp)_Synthase.
 IPR000924; Glu/Gln-tRNA-synth_Ib.
 IPR020058; Glu/Gln-tRNA-synth_Ib_cat-dom.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00749; tRNA-synt_1c 

SMART

  

PROSITE

 PS00178; AA_TRNA_LIGASE_I 

PRINTS

 PR00987; TRNASYNTHGLU.