CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000191
UniProt Accession
Genbank Protein ID
 U72995 
Genbank Nucleotide ID
Protein Name
 MAP kinase-activating death domain protein 
Protein Synonyms/Alias
 Rab3 GDP/GTP exchange factor 
Gene Name
 Madd 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
540RQTPFAEKLARTQAVubiquitination[1]
961LRVFVLSKLSRAVQSacetylation[2]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). 
Sequence Annotation
 DOMAIN 24 97 UDENN.
 DOMAIN 171 401 DENN.
 DOMAIN 484 557 dDENN.
 DOMAIN 1295 1370 Death.
 MOD_RES 812 812 Phosphoserine.
 MOD_RES 817 817 Phosphoserine (By similarity).
 MOD_RES 819 819 Phosphoserine (By similarity).
 MOD_RES 900 900 Phosphoserine (By similarity).
 MOD_RES 1038 1038 Phosphoserine (By similarity).
 MOD_RES 1040 1040 Phosphothreonine (By similarity).  
Keyword
 Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1602 AA 
Protein Sequence
MVQKKFCPRL LDYLVIVGAR HPSSDSVAQT PELLRRYPLE DHPEFPLPPD VVFFCQPEGC 60
LSVRQRRMSL RDDTSFVFTL TDKDTGVTRY GICVNFYRSF QKRMPKEKAE GGAGPRGKEG 120
AHAPCASEEA ATESSESGST LQPPSADSTP DVNQSPRGKR RAKAGNRSRN STLTSLCVLS 180
HYPFFSTFRE CLYTLKRLVD CCSERLLGKK PGIPRGVQRD TMWRIFTGSL LVEEKSSALL 240
HDLREIEAWI YRLLRSPVPV SGQKRVDIEV LPQEVQQALT FALPDPSRFT LVDFPLHLPL 300
ELLGVDACLQ VLTCILLEHK VVLQSRDYNA LSMSVMAFVA MIYPLEYMFP VIPLLPTCMA 360
SAEQLLLAPT PYIIGVPASF FLYKLDFKMP DDVWLVDLDS NRVIAPTNAE VLPILPEPES 420
LELKKHLKQA LASMSLNTQP ILNLEKFHEG QETPLLLGRF SNDLQSTPST EFNPLIYGND 480
VDSVDVATRV AMVRFFNSAN VLQGFQMHTR TLRLFPRPVV AFQAGSFLAS RPRQTPFAEK 540
LARTQAVEYF GEWILNPSNY AFQRIHNNTF DPALIGDKPK WYAHQLQPIH YRVYDSNSQL 600
AEALSVPPER DSESDPTDDS GSDSMDYDDS SSSYSSLGDF VSEMMKCDIN GDTPNVDPLT 660
HAALGDASEV EIDELQPQKE GEEPGPDSEN SQENLPLRSS SSTTASSSPS TIVHGAHSEP 720
ADSTEVGDKA ATGISKPLPP VPPSICKSTV DRRQTETGEG SVCQRTYDHP YFEPQYGSPA 780
EEDDDEQGES YTPRFSQHAS GSRAQKLLRP NSLKLASDSD AESDSRASSP NSTVSNNSTE 840
GFGGIMSFAS SLYRNHSTSF SLSNLTLPTK GAREKTTPFP SLKGNRRALV DQKSSVIKHS 900
PTVKREPPSP QGRSSNSSEN QQFLKEVVHS VLDGQGVGWL NMKKVRRLLE SEQLRVFVLS 960
KLSRAVQSED DARQDVIQDV EISRKVYKGM LDLLKCTVLS LEQSYAHAGL GGMASIFGLL 1020
EIAQTHYYSK EPDKRKRSPT ENVNTPVGKD PGLAGRGDPK AMAQLRVPQL GPRAPSATGR 1080
GPKELDTRSL KEENFVASVG PEVIKPVFDL GETEEKKSQI SADSGVSLAS ASQRTDQDSV 1140
IGVSPAVMIR SSSQDSEVSN SSGETLGADS DLSSNAGDGP GGEGSAHLAS SRATLSDSEI 1200
ETNSATSTIF GKAHSLKPKE KPASSPVRSS EDVSQRVYLY EGLLGRDKGS MWDQLEDAAM 1260
ETFSISKERS TLWDQMQFWE DAFLDAVMLE REGMGMDQGP QEMIDRYLSL GEHDRKRLED 1320
DEDRLLATLL HNLISYMLLM KVNKNDIRKK VRRLMGKSHV GLVYSQQINE VLDQLTNLNG 1380
RDLSIRSSGS RHMKKQTFVV HAGTDTNGDI FFMEVCDDCV VLRSNIGTVY ERWWYEKLIN 1440
MTYCPKTKVL CLWRRNGSET QLNKFYTKKC RELYYCVKDS MERAAARQQS IKPGPELGGE 1500
FPVQDMKTGE GGLLQVTLEG INLKFMHNQV FIELNHIKKC NTVRGVFVLE EFVPEIKEVV 1560
SHKYKTPMAH EICYSVLCLF SYVAAVRSSE EDLRTPPRPV SS 1602 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0017112; F:Rab guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
 GO:0000187; P:activation of MAPK activity; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051726; P:regulation of cell cycle; ISS:UniProtKB.
 GO:1902041; P:regulation of extrinsic apoptotic signaling pathway via death domain receptors; ISS:UniProtKB. 
Interpro
 IPR005112; dDENN_dom.
 IPR001194; DENN_dom.
 IPR005113; uDENN_dom. 
Pfam
 PF03455; dDENN
 PF02141; DENN
 PF03456; uDENN 
SMART
 SM00801; dDENN
 SM00799; DENN
 SM00800; uDENN 
PROSITE
 PS50947; DDENN
 PS50017; DEATH_DOMAIN
 PS50211; DENN
 PS50946; UDENN 
PRINTS