CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031478
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U5 small nuclear ribonucleoprotein 200 kDa helicase 
Protein Synonyms/Alias
 cDNA FLJ56901, highly similar to U5 small nuclear ribonucleoprotein 200 kDa helicase (EC 3.6.1.-) 
Gene Name
 SNRNP200 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71TRLLSMAKPVYHAITubiquitination[1, 2, 3]
84ITKHSPKKPVIVFVPubiquitination[4]
122RFLHCTEKDLIPYLEacetylation[4]
122RFLHCTEKDLIPYLEubiquitination[3, 4]
130DLIPYLEKLSDSTLKubiquitination[5]
238IMCQGSKKDFFKKFLubiquitination[3]
275VTKTIENKQDAVDYLubiquitination[6]
488WSKDSYLKQLPHFTSubiquitination[2]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome; Ribonucleoprotein; Viral nucleoprotein; Virion. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 595 AA 
Protein Sequence
MRYISSQIER PIRIVALSSS LSNAKDVAHW LGCSATSTFN FHPNVRPVPL ELHIQGFNIS 60
HTQTRLLSMA KPVYHAITKH SPKKPVIVFV PSRKQTRLTA IDILTTCAAD IQRQRFLHCT 120
EKDLIPYLEK LSDSTLKETL LNGVGYLHEG LSPMERRLVE QLFSSGAIQV VVASRSLCWG 180
MNVAAHLVII MDTQYYNGKI HAYVDYPIYD VLQMVGHANR PLQDDEGRCV IMCQGSKKDF 240
FKKFLYEPLP VESHLDHCMH DHFNAEIVTK TIENKQDAVD YLTWTFLYRR MTQNPNYYNL 300
QGISHRHLSD HLSELVEQTL SDLEQSKCIS IEDEMDVAPL NLGMIAAYYY INYTTIELFS 360
MSLNAKTKVR GLIEIISNAA EYENIPIRHH EDNLLRQLAQ KVPHKLNNPK FNDPHVKTNL 420
LLQAHLSRMQ LSAELQSDTE EILSKAIRLI QACVDVLSSN GWLSPALAAM ELAQMVTQAM 480
WSKDSYLKQL PHFTSEHIKR CTDKGVESVF DIMEMEDEER NALLQLTDSQ IADVARFCNR 540
YPNIELSYEV VDKDSIRSGG PVVVLVQLER EEEVTGPVIA PLFPQFRAGR SGSRL 595 
Gene Ontology
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:UniProtKB-KW. 
Interpro
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR004179; Sec63-dom. 
Pfam
 PF02889; Sec63 
SMART
 SM00490; HELICc
 SM00611; SEC63
 SM00973; Sec63 
PROSITE
 PS51194; HELICASE_CTER 
PRINTS