CPLM-009649

Genbank Nucleotide ID

Ras-related C3 botulinum toxin substrate 1

Protein Synonyms/Alias

Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1

Homo sapiens (Human)

Position	Peptide	Type	References
152	IEKLKEKKLTPITYP	ubiquitination	[1]
166	PQGLAMAKEIGAVKY	ubiquitination	[1, 2]
172	AKEIGAVKYLECSAL	ubiquitination	[2]

[1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.

NP_BIND 10 17 GTP (By similarity).
NP_BIND 57 61 GTP (By similarity).
NP_BIND 115 118 GTP (By similarity).
MOTIF 32 40 Effector region (Potential).
MOD_RES 32 32 O-AMP-tyrosine; by Haemophilus IbpA.
MOD_RES 35 35 O-AMP-threonine; by Vibrio VopS.
MOD_RES 39 39 ADP-ribosylasparagine; by botulinum toxin
MOD_RES 189 189 Cysteine methyl ester.
LIPID 189 189 S-geranylgeranyl cysteine.
CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly)

3D-structure; ADP-ribosylation; Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; GTP-binding; Isopeptide bond; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Polymorphism; Prenylation; Reference proteome; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0019897; C:extrinsic to plasma membrane; IEA:Compara.
GO:0000139; C:Golgi membrane; IEA:Compara.
GO:0030027; C:lamellipodium; IEA:Compara.
GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
GO:0001891; C:phagocytic cup; IEA:Compara.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0032587; C:ruffle membrane; IEA:Compara.
GO:0005802; C:trans-Golgi network; IDA:FlyBase.
GO:0005525; F:GTP binding; IDA:UniProtKB.
GO:0003924; F:GTPase activity; TAS:UniProtKB.
GO:0030041; P:actin filament polymerization; TAS:UniProtKB.
GO:0048532; P:anatomical structure arrangement; IEA:Compara.
GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
GO:0002093; P:auditory receptor cell morphogenesis; IEA:Compara.
GO:0007411; P:axon guidance; TAS:Reactome.
GO:0045453; P:bone resorption; IEA:Compara.
GO:0048870; P:cell motility; IDA:UniProtKB.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0045216; P:cell-cell junction organization; IEA:Compara.
GO:0007160; P:cell-matrix adhesion; NAS:BHF-UCL.
GO:0021799; P:cerebral cortex radially oriented cell migration; IEA:Compara.
GO:0090103; P:cochlea morphogenesis; IEA:Compara.
GO:0048813; P:dendrite morphogenesis; IEA:Compara.
GO:0071542; P:dopaminergic neuron differentiation; IEA:Compara.
GO:0021831; P:embryonic olfactory bulb interneuron precursor migration; IEA:Compara.
GO:0043652; P:engulfment of apoptotic cell; IEA:Compara.
GO:0003382; P:epithelial cell morphogenesis; IEA:Compara.
GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
GO:0006972; P:hyperosmotic response; IEA:Compara.
GO:0006954; P:inflammatory response; TAS:ProtInc.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
GO:0030032; P:lamellipodium assembly; IMP:UniProtKB.
GO:0051668; P:localization within membrane; IMP:BHF-UCL.
GO:0002551; P:mast cell chemotaxis; IEA:Compara.
GO:0032707; P:negative regulation of interleukin-23 production; IDA:BHF-UCL.
GO:0048261; P:negative regulation of receptor-mediated endocytosis; TAS:UniProtKB.
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
GO:0030168; P:platelet activation; TAS:Reactome.
GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
GO:0010592; P:positive regulation of lamellipodium assembly; IDA:MGI.
GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Compara.
GO:0035025; P:positive regulation of Rho protein signal transduction; TAS:UniProtKB.
GO:0030334; P:regulation of cell migration; IMP:UniProtKB.
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
GO:0010310; P:regulation of hydrogen peroxide metabolic process; TAS:BHF-UCL.
GO:0060263; P:regulation of respiratory burst; IDA:BHF-UCL.
GO:0097178; P:ruffle assembly; IEA:Compara.
GO:0031529; P:ruffle organization; TAS:UniProtKB.
GO:0007264; P:small GTPase mediated signal transduction; IEA:Compara.
GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Compara.
GO:0031295; P:T cell costimulation; TAS:Reactome.
GO:0016032; P:viral reproduction; TAS:Reactome.
GO:0060071; P:Wnt receptor signaling pathway, planar cell polarity pathway; IEA:Compara.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR003578; Small_GTPase_Rho.

PR00449; RASTRNSFRMNG.