CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014206
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA polymerase-associated protein LEO1 
Protein Synonyms/Alias
  
Gene Name
 Leo1 
Gene Synonyms/Alias
 Gm185 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
365RIEVEIPKVNTDLGNubiquitination[1]
416GRTRLKLKVENTIRWubiquitination[1]
487LQGQAVFKTKLTFRPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 10 10 Phosphoserine (By similarity).
 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 151 151 Phosphoserine (By similarity).
 MOD_RES 154 154 Phosphoserine (By similarity).
 MOD_RES 162 162 Phosphoserine (By similarity).
 MOD_RES 171 171 Phosphoserine (By similarity).
 MOD_RES 179 179 Phosphoserine (By similarity).
 MOD_RES 189 189 Phosphothreonine (By similarity).
 MOD_RES 198 198 Phosphoserine (By similarity).
 MOD_RES 206 206 Phosphoserine (By similarity).
 MOD_RES 213 213 Phosphoserine (By similarity).
 MOD_RES 221 221 Phosphoserine (By similarity).
 MOD_RES 230 230 Phosphoserine (By similarity).
 MOD_RES 239 239 Phosphoserine (By similarity).
 MOD_RES 247 247 Phosphoserine.
 MOD_RES 255 255 Phosphoserine.
 MOD_RES 278 278 Phosphoserine.
 MOD_RES 280 280 Phosphoserine.
 MOD_RES 295 295 Phosphoserine.
 MOD_RES 297 297 Phosphoserine.
 MOD_RES 301 301 Phosphoserine (By similarity).
 MOD_RES 608 608 Phosphoserine.
 MOD_RES 609 609 Phosphoserine.
 MOD_RES 611 611 Phosphoserine.
 MOD_RES 615 615 Phosphoserine.
 MOD_RES 631 631 Phosphoserine.
 MOD_RES 659 659 Phosphoserine.  
Keyword
 Acetylation; Activator; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 667 AA 
Protein Sequence
MADMEDLFGS EAESEAERKD SESESDSDSD QDNGASGSNA SGSESDQDDR GDSGQPSNKE 60
LFGDDSEEEG ASHHSGSDNH SERSDNRSEA SERSDHEDNE PSDEDQHSGS EAHNDDDDEG 120
HRSDEGSRHS EAEGSEKAQS DDEKWDGEDK SDQSDDEKLQ NSDDEDREQG SDEDKLQNSD 180
DDEEKMQNTD DEDRAQISDD DRQQLSEEEK GNSDDEHPVA SDNDEEKQNS DDEDQPQVSD 240
EEKMQNSDDE RPQVSDEDGR RSDGEEEQDQ KSESARGSDS EDEVLRLKRK NAIPSDSEAD 300
SDTEVPKDNN GTMDLFGGAD DISSGSDGED KPPTPGQPVD ENGLPQDQQE EEPIPETRIE 360
VEIPKVNTDL GNDLYFVKLP NFLSVEPRPF DPQYYEDEFE DEEMLDEEGR TRLKLKVENT 420
IRWRIRRDEE GNEIKESNAR IVKWSDGSMS LHLGNEVFDV YKAPLQGDHN HLFIRQGTGL 480
QGQAVFKTKL TFRPHSTDSA THRKMTLSLA DRCSKTQKIR ILPMAGRDPE CQRTEMIKKE 540
EERLRASIRR ESQQRRMREK QHQRGLSASY LEPDRYDEEE EGEESVSLAA IKNRYKGGIR 600
EERARIYSSD SDEGSEEDKA QRLLKAKKLN SDEEGESSGK RKAEDDDKAN KKHKKYVISD 660
EEEEEDD 667 
Gene Ontology
 GO:0016593; C:Cdc73/Paf1 complex; ISS:UniProtKB.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0001711; P:endodermal cell fate commitment; IMP:UniProtKB.
 GO:0033523; P:histone H2B ubiquitination; IEA:Compara.
 GO:0010390; P:histone monoubiquitination; IEA:Compara.
 GO:0006378; P:mRNA polyadenylation; ISS:UniProtKB.
 GO:0045638; P:negative regulation of myeloid cell differentiation; ISS:UniProtKB.
 GO:0031442; P:positive regulation of mRNA 3'-end processing; ISS:UniProtKB.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0019827; P:stem cell maintenance; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR007149; Leo1. 
Pfam
 PF04004; Leo1 
SMART
  
PROSITE
  
PRINTS