UniProt Accession

 DHB2_MOUSE; P51658; O08898 

Genbank Protein ID

 Y09517; X95685 

Genbank Nucleotide ID

 CAA70706.1; CAA64982.1 

Protein Name

 Estradiol 17-beta-dehydrogenase 2 

Protein Synonyms/Alias

 17-beta-hydroxysteroid dehydrogenase type 2; 17-beta-HSD 2; Testosterone 17-beta-dehydrogenase 

Gene Name

 Hsd17b2 

Gene Synonyms/Alias

 Edh17b2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
101	GFGHGLAKHLDKLGF	ubiquitination	[1]
142	VLQMDVTKPEQIKDA	ubiquitination	[1]
147	VTKPEQIKDAHSKVT	acetylation	[2]
147	VTKPEQIKDAHSKVT	ubiquitination	[1]
152	QIKDAHSKVTEKIQD	acetylation	[3]
262	GVKVVTIKPGGFKTN	ubiquitination	[1]
279	GSQDIWDKMEKEILD	acetylation	[2, 3]
279	GSQDIWDKMEKEILD	ubiquitination	[1]
290	EILDHFSKDIQENYG	ubiquitination	[1]
305	QDYVHTQKLIIPTLK	ubiquitination	[1]
312	KLIIPTLKERSNPDI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity). 

Sequence Annotation

 NP_BIND 83 112 NAD (By similarity).
 ACT_SITE 233 233 Proton acceptor (By similarity).
 BINDING 220 220 Substrate (By similarity).  

Keyword

 Complete proteome; Lipid biosynthesis; Lipid metabolism; Membrane; NAD; Oxidoreductase; Reference proteome; Signal-anchor; Steroid biosynthesis; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 381 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:EC.
 GO:0050327; F:testosterone 17-beta-dehydrogenase (NAD+) activity; IEA:Compara.
 GO:0047035; F:testosterone dehydrogenase (NAD+) activity; IEA:EC.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0001890; P:placenta development; IMP:MGI.
 GO:0032526; P:response to retinoic acid; IEA:Compara.
 GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.