CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003822
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 cAMP-dependent protein kinase type I-alpha regulatory subunit 
Protein Synonyms/Alias
 Tissue-specific extinguisher 1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed 
Gene Name
 PRKAR1A 
Gene Synonyms/Alias
 PKR1; PRKAR1; TSE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24ECELYVQKHNIQALLubiquitination[1, 2]
59EYFERLEKEEAKQIQubiquitination[2]
63RLEKEEAKQIQNLQKubiquitination[2, 3, 4]
70KQIQNLQKAGTRTDSubiquitination[1, 2, 3, 4]
92PPPNPVVKGRRRRGAubiquitination[1, 2, 3, 4]
123KVIPKDYKTMAALAKubiquitination[2, 3]
130KTMAALAKAIEKNVLubiquitination[2]
134ALAKAIEKNVLFSHLubiquitination[1, 5]
222VKAKTNVKLWGIDRDubiquitination[1, 2, 4]
244GSTLRKRKMYEEFLSubiquitination[3, 4]
252MYEEFLSKVSILESLubiquitination[1, 3, 4]
261SILESLDKWERLTVAacetylation[6]
261SILESLDKWERLTVAubiquitination[1, 2, 3, 4, 5, 7, 8, 9]
349RGPLKCVKLDRPRFEubiquitination[1, 3, 9]
367GPCSDILKRNIQQYNubiquitination[2, 10]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [7] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. 
Sequence Annotation
 NP_BIND 137 254 cAMP 1.
 NP_BIND 255 381 cAMP 2.
 REGION 1 136 Dimerization and phosphorylation.
 MOTIF 96 100 Pseudophosphorylation motif.
 BINDING 202 202 cAMP 1.
 BINDING 211 211 cAMP 1.
 BINDING 326 326 cAMP 2.
 BINDING 335 335 cAMP 2.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 77 77 Phosphoserine (By similarity).
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 101 101 Phosphoserine (By similarity).
 DISULFID 18 18 Interchain (with C-39) (By similarity).
 DISULFID 39 39 Interchain (with C-18) (By similarity).  
Keyword
 Acetylation; cAMP; cAMP-binding; Cell membrane; Complete proteome; Cushing syndrome; Direct protein sequencing; Disease mutation; Disulfide bond; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 381 AA 
Protein Sequence
MESGSTAASE EARSLRECEL YVQKHNIQAL LKDSIVQLCT ARPERPMAFL REYFERLEKE 60
EAKQIQNLQK AGTRTDSRED EISPPPPNPV VKGRRRRGAI SAEVYTEEDA ASYVRKVIPK 120
DYKTMAALAK AIEKNVLFSH LDDNERSDIF DAMFSVSFIA GETVIQQGDE GDNFYVIDQG 180
ETDVYVNNEW ATSVGEGGSF GELALIYGTP RAATVKAKTN VKLWGIDRDS YRRILMGSTL 240
RKRKMYEEFL SKVSILESLD KWERLTVADA LEPVQFEDGQ KIVVQGEPGD EFFIILEGSA 300
AVLQRRSENE EFVEVGRLGP SDYFGEIALL MNRPRAATVV ARGPLKCVKL DRPRFERVLG 360
PCSDILKRNI QQYNSFVSLS V 381 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IDA:LIFEdb.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; TAS:ProtInc.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0034199; P:activation of protein kinase A activity; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0006833; P:water transport; TAS:Reactome. 
Interpro
 IPR002373; cAMP/cGMP_kin.
 IPR012198; cAMP_dep_PK_reg_su.
 IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF02197; RIIa 
SMART
 SM00100; cNMP
 SM00394; RIIa 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 
PRINTS
 PR00103; CAMPKINASE.