UniProt Accession

 NADE_BACSU; P08164 

Genbank Protein ID

 M15811; D50453; AL009126 

Genbank Nucleotide ID

 AAA22635.1; BAA08947.1; CAB12107.1 

Protein Name

 NH(3)-dependent NAD(+) synthetase 

Protein Synonyms/Alias

 General stress protein 38; GSP38; Spore outgrowth factor B; Sporulation protein OutB 

Gene Name

 nadE 

Gene Synonyms/Alias

 outB; BSU03130 

Created Date

 July 27, 2013 

Organism

 Bacillus subtilis (strain 168) 

NCBI Taxa ID

 224308 

Lysine Modification

Position	Peptide	Type	References
195	RQGRTLLKELGAPER	acetylation	[1]

Reference

 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 

Functional Description

 Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction. 

Sequence Annotation

 NP_BIND 45 52 ATP.
 NP_BIND 168 178 NAD.
 BINDING 33 33 NAD.
 BINDING 79 79 ATP.
 BINDING 85 85 ATP.
 BINDING 138 138 NAD.
 BINDING 158 158 ATP.
 BINDING 209 209 ATP.
 BINDING 224 224 NAD.
 BINDING 259 259 NAD.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; NAD; Nucleotide-binding; Phosphoprotein; Reference proteome; Sporulation; Stress response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 272 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
 GO:0008795; F:NAD+ synthase activity; IEA:HAMAP.
 GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. 

Interpro

 IPR022310; NAD/GMP_synthase.
 IPR003694; NAD_synthase.
 IPR022926; NH(3)-dep_NAD(+)_synth.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF02540; NAD_synthase 

SMART

  

PROSITE

  

PRINTS