UniProt Accession

 PORED_HUMAN; Q9H8P0; Q4W5Q6 

Genbank Protein ID

 AK023414; CR457312; AC064824; CH471057; BC002480 

Genbank Nucleotide ID

 BAB14568.1; CAG33593.1; AAY40904.1; EAX05465.1; AAH02480.1 

Protein Name

 Polyprenol reductase 

Protein Synonyms/Alias

 3-oxo-5-alpha-steroid 4-dehydrogenase 3; Steroid 5-alpha-reductase 2-like; Steroid 5-alpha-reductase 3; S5AR 3; SR type 3 

Gene Name

 SRD5A3 

Gene Synonyms/Alias

 SRD5A2L 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	LIRYGKTKCGEPSRP	ubiquitination	[1]
193	RNAYITGKNLLMQAR	acetylation	[2]
193	RNAYITGKNLLMQAR	ubiquitination	[3]
229	VILGNLRKNKAGVVI	acetylation	[2]
302	SHQFYKSKFVSYPKH	ubiquitination	[1]
311	VSYPKHRKAFLPFLF	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT). 

Sequence Annotation

  

Keyword

 Cataract; Complete proteome; Congenital disorder of glycosylation; Endoplasmic reticulum; Membrane; Mental retardation; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 318 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IDA:UniProtKB.
 GO:0047751; F:cholestenone 5-alpha-reductase activity; IEA:EC.
 GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IDA:UniProtKB.
 GO:0006702; P:androgen biosynthetic process; TAS:Reactome.
 GO:0019348; P:dolichol metabolic process; IDA:UniProtKB.
 GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; IMP:UniProtKB.
 GO:0016095; P:polyprenol catabolic process; IDA:UniProtKB.
 GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001104; 3-oxo-5_a-steroid_4-DH_C. 

Pfam

 PF02544; Steroid_dh 

SMART

  

PROSITE

 PS50244; S5A_REDUCTASE 

PRINTS