UniProt Accession

 SAR1A_HUMAN; Q9NR31 

Genbank Protein ID

 AF261717; AL136724; AY008268; BC003658 

Genbank Nucleotide ID

 AAF81741.1; CAB66658.1; AAG16638.1; AAH03658.1 

Protein Name

 GTP-binding protein SAR1a 

Protein Synonyms/Alias

 COPII-associated small GTPase 

Gene Name

 SAR1A 

Gene Synonyms/Alias

 SAR1; SARA; SARA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
27	GLYKKSGKLVFLGLD	ubiquitination	[1, 2]
146	TDAISEEKLREIFGL	ubiquitination	[3, 4]
166	GKGNVTLKELNARPM	ubiquitination	[2, 4]
182	VFMCSVLKRQGYGEG	ubiquitination	[4]

Reference

 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). 

Sequence Annotation

 NP_BIND 32 39 GTP (By similarity).
 NP_BIND 75 78 GTP (By similarity).
 NP_BIND 134 137 GTP (By similarity).
 MOD_RES 139 139 Phosphothreonine.  

Keyword

 3D-structure; Complete proteome; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; GTP-binding; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 198 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016529; C:sarcoplasmic reticulum; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; NAS:UniProtKB.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; NAS:UniProtKB.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR006689; Small_GTPase_ARF/SAR.
 IPR006687; Small_GTPase_SAR1. 

Pfam

 PF00025; Arf 

SMART

 SM00178; SAR 

PROSITE

 PS51422; SAR1 

PRINTS

 PR00328; SAR1GTPBP.