CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027254
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine desulfurase, mitochondrial 
Protein Synonyms/Alias
 Nitrogen fixation gene 1 (S. cerevisiae), isoform CRA_b 
Gene Name
 Nfs1 
Gene Synonyms/Alias
 mCG_5229 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
159VTTQTEHKCVLDSCRacetylation[1, 2, 3]
228GQICSSRKVYFHTDAacetylation[2]
427EVDYTAEKCIHHVKRacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 459 AA 
Protein Sequence
MVGSVAGNML LRAAWRRASL AATSLALGRS SVPTRGLRLR VVDHGPHSPV HSEAEAVLRP 60
LYMDVQATTP LDPRVLDAML PYLVNYYGNP HSRTHAYGWE SEAAMERARQ QVASLIGADP 120
REIIFTSGAT ESNNIAIKGV ARFYRSRKKH LVTTQTEHKC VLDSCRSLEA EGFRVTYLPV 180
QKSGIIDLKE LEAAIQPDTS LVSVMTVNNE IGVKQPIAEI GQICSSRKVY FHTDAAQAVG 240
KIPLDVNDMK IDLMSISGHK LYGPKGVGAI YIRRRPRVRV EALQSGGGQE RGMRSGTVPT 300
PLVVGLGAAC ELAQQEMEYD HKRISKLAER LVQNIMKNLP DVVMNGDPKQ HYPGCINLSF 360
AYVEGESLLM ALKDVALSSG SACTSASLEP SYVLRAIGTD EDLAHSSIRF GIGRFTTEEE 420
VDYTAEKCIH HVKRLREMSP LWEMVQDGID LKSIKWTQH 459 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0031071; F:cysteine desulfurase activity; IEA:Compara.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006534; P:cysteine metabolic process; IEA:InterPro.
 GO:0018283; P:iron incorporation into metallo-sulfur cluster; IEA:Compara. 
Interpro
 IPR000192; Aminotrans_V/Cys_dSase.
 IPR020578; Aminotrans_V_PyrdxlP_BS.
 IPR010240; Cys_deSase.
 IPR016454; Cysteine_dSase_NifS.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 
Pfam
 PF00266; Aminotran_5 
SMART
  
PROSITE
 PS00595; AA_TRANSFER_CLASS_5 
PRINTS