CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037134
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Eukaryotic translation initiation factor 4 gamma 1 
Protein Synonyms/Alias
  
Gene Name
 EIF4G1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1020WNTVPISKGSRPIDTubiquitination[1]
1032IDTSRLTKITKPGSIubiquitination[2, 3, 4]
1149ATKRSFSKEVEERSRubiquitination[3]
1205LSEEELEKKSKAIIEacetylation[4]
1365REAGLSWKEFLPEGQubiquitination[5]
1541QGKGVALKSVTAFFKubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1560 AA 
Protein Sequence
MNTPSQPRQH FYPSRAQPPS SAASRVQSAA PARPGPAAHV YPAGSQVMMI PSQISYPASQ 60
GAYYIPGQGR STYVVPTQQY PVQPGAPGFY PGASPTEFGT YAGAYYPAQG VQQFPTGVAP 120
TPVLMNQPPQ IAPKRERKTI RIRDPNQGGK DITEEIMSGA RTASTPTPPQ TGGGLEPQAN 180
GETPQVAVIV RPDDRSQGAI IADRPGLPGP EHSPSESQPS SPSPTPSPSP VLEPGSEPNL 240
AVLSIPGDTM TTIQMSVEES TPISRETGEP YRLSPEPTPL AEPILEVEVT LSKPVPESEF 300
SSSPLQAPTP LASHTVEIHE PNGMVPSEDL EPEVESSPEL APPPACPSES PVPIAPTAQP 360
EELLNGAPSP PAVDLSPVSE PEEQAKEVTA SMAPPTIPSA TPATAPSATS PAQEEEMEEE 420
EEEEEGEAGE AGEAESEKGG EELLPPESTP IPANLSQNLE AAAATQVAVS VPKRRRKIKE 480
LNKKEAVGDL LDAFKEANPA VPEVENQPPA GSNPGPESEG SGVPPRPEEA DETWDSKEDK 540
IHNAENIQPG EQKYEYKSDQ WKPLNLEEKK RYDREFLLGF QFIFASMQKP EGLPHISDVV 600
LDKANKTPLR PLDPTRLQGI NCGPDFTPSF ANLGRTTLST RGPPRGGPGG ELPRGPQAGL 660
GPRRSQQGPR KEPRKIIATV LMTEDIKLNK AEKAWKPSSK RTAADKDRGE EDADGSKTQD 720
LFRRVRSILN KLTPQMFQQL MKQVTQLAID TEERLKGVID LIFEKAISEP NFSVAYANMC 780
RCLMALKVPT TEKPTVTVNF RKLLLNRCQK EFEKDKDDDE VFEKKQKEMD EAATAEERGR 840
LKEELEEARD IARRRSLGNI KFIGELFKLK MLTEAIMHDC VVKLLKNHDE ESLECLCRLL 900
TTIGKDLDFE KAKPRMDQYF NQMEKIIKEK KTSSRIRFML QDVLDLRGSN WVPRRGDQGP 960
KTIDQIHKEA EMEEHREHIK VQQLMAKGSD KRRGGPPGPP ISRGLPLVDD GGWNTVPISK 1020
GSRPIDTSRL TKITKPGSID SNNQLFAPGG RLSWGKGSSG GSGAKPSDAA SEAARPATST 1080
LNRFSALQQA VPTESTDNRR VVQRSSLSRE RGEKAGDRGD RLERSERGGD RGDRLDRART 1140
PATKRSFSKE VEERSRERPS QPEGLRKAAS LTEDRDRGRD AVKREAALPP VSPLKAALSE 1200
EELEKKSKAI IEEYLHLNDM KEAVQCVQEL ASPSLLFIFV RHGVESTLER SAIAREHMGQ 1260
LLHQLLCAGH LSTAQYYQGL YEILELAEDM EIDIPHVWLY LAELVTPILQ EGGVPMGELF 1320
REITKPLRPL GKAASLLLEI LGLLCKSMGP KKVGTLWREA GLSWKEFLPE GQDIGAFVAE 1380
QKVEYTLGEE SEAPGQRALP SEELNRQLEK LLKEGSSNQR VFDWIEANLS EQQIVSNTLV 1440
RALMTAVCYS AIIFETPLRV DVAVLKARAK LLQKYLCDEQ KELQALYALQ ALVVTLEQPP 1500
NLLRMFFDAL YDEDVVKEDA FYSWESSKDP AEQQGKGVAL KSVTAFFKWL REAEEESDHN 1560 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR003891; Initiation_fac_eIF4g_MI.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3.
 IPR003307; W2_domain. 
Pfam
 PF02847; MA3
 PF02854; MIF4G
 PF02020; W2 
SMART
 SM00515; eIF5C
 SM00544; MA3
 SM00543; MIF4G 
PROSITE
 PS51366; MI
 PS51363; W2 
PRINTS