UniProt Accession

 ACSL1_MOUSE; P41216; Q6GTG6; Q9DBK5 

Genbank Protein ID

 U15977; AK004897; AK145900; AK149406; AK152772; AK153050; AK161189; AK168078; BC056644 

Genbank Nucleotide ID

 AAA52193.1; BAB23652.1; BAE26736.1; BAE28854.1; BAE31484.1; BAE31678.1; BAE36230.1; BAE40051.1; AAH56644.1 

Protein Name

 Long-chain-fatty-acid--CoA ligase 1 

Protein Synonyms/Alias

 Long-chain acyl-CoA synthetase 1; LACS 1 

Gene Name

 Acsl1 

Gene Synonyms/Alias

 Acsl2; Facl2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
52	ATRPKALKPPCDLSM	acetylation	[1]
81	SAVLEDDKLLVYYYD	acetylation	[1]
208	ADKPEKAKLLLEGVE	acetylation	[1]
415	RNNSLWDKLIFHKIQ	acetylation	[1]
420	WDKLIFHKIQSSLGG	acetylation	[1]
518	VKGANVFKGYLKDPA	acetylation	[1]
533	RTAEALDKDGWLHTG	acetylation	[1]
544	LHTGDIGKWLPNGTL	acetylation	[1, 2]
629	FEELCRNKDINKAIL	acetylation	[1]
633	CRNKDINKAILDDLL	acetylation	[1]
644	DDLLKLGKEAGLKPF	acetylation	[1]
649	LGKEAGLKPFEQVKG	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647] 

Functional Description

 Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates (By similarity). 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 9 9 Nitrated tyrosine (By similarity).
 MOD_RES 85 85 Phosphotyrosine (By similarity).
 MOD_RES 86 86 Nitrated tyrosine (By similarity).
 MOD_RES 544 544 N6-acetyllysine.
 MOD_RES 633 633 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nitration; Nucleotide-binding; Peroxisome; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 699 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005778; C:peroxisomal membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004467; F:long-chain fatty acid-CoA ligase activity; IEA:EC.
 GO:0033211; P:adiponectin-mediated signaling pathway; IMP:MGI.
 GO:0008610; P:lipid biosynthetic process; IEA:Compara.
 GO:0044539; P:long-chain fatty acid import; IEA:Compara.
 GO:0071902; P:positive regulation of protein serine/threonine kinase activity; IMP:MGI.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0034201; P:response to oleic acid; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0006641; P:triglyceride metabolic process; IEA:Compara.
 GO:0042178; P:xenobiotic catabolic process; IEA:Compara. 

Interpro

 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig. 

Pfam

 PF00501; AMP-binding 

SMART

  

PROSITE

 PS00455; AMP_BINDING 

PRINTS