CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024542
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin-related protein 2 
Protein Synonyms/Alias
 Actin-like protein 2 
Gene Name
 ACTR2 
Gene Synonyms/Alias
 ARP2 
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
46PIIRSTTKVGNIEIKubiquitination[1]
118EPPMNPTKNREKIVEubiquitination[1]
299DTRSEFYKHIVLSGGubiquitination[1]
336VLKGDVEKLSKFKIRubiquitination[1]
368LADIMKDKDNFWMTRubiquitination[1]
388KGVRVLEKLGVTVR*ubiquitination[1]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. 
Sequence Annotation
 NP_BIND 160 162 ATP.
 NP_BIND 214 218 ATP.
 NP_BIND 305 310 ATP.
 MOD_RES 299 299 N6-acetyllysine (By similarity).
 MOD_RES 322 322 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Actin-binding; ATP-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 394 AA 
Protein Sequence
MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI EIKDLMVGDE 60
ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI DTRNCKILLT EPPMNPTKNR 120
EKIVEVMFET YQFSGVYVAI QAVLTLYAQG LLTGVVVDSG DGVTHICPVY EGFSLPHLTR 180
RLDIAGRDIT RYLIKLLLLR GYAFNHSADF ETVRMIKEKL CYVGYNIEQE QKLALETTVL 240
VESYTLPDGR IIKVGGERFE APEALFQPHL INVEGVGVAE LLFNTIQAAD IDTRSEFYKH 300
IVLSGGSTMY PGLPSRLERE LKQLYLERVL KGDVEKLSKF KIRIEDPPRR KHMVFLGGAV 360
LADIMKDKDN FWMTRQEYQE KGVRVLEKLG VTVR 394 
Gene Ontology
 GO:0005885; C:Arp2/3 protein complex; IEA:InterPro.
 GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro. 
Interpro
 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS.
 IPR027306; Arp2. 
Pfam
 PF00022; Actin 
SMART
 SM00268; ACTIN 
PROSITE
 PS01132; ACTINS_ACT_LIKE 
PRINTS
 PR00190; ACTIN.