CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022361
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 H/ACA ribonucleoprotein complex subunit 1 
Protein Synonyms/Alias
 Nucleolar protein family A member 1; snoRNP protein GAR1 
Gene Name
 GAR1 
Gene Synonyms/Alias
 NOLA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
134VKLSENMKASSFKKLubiquitination[1]
143SSFKKLQKFYIDPYKubiquitination[1, 2]
150KFYIDPYKLLPLQRFubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. 
Sequence Annotation
 REGION 4 57 RGG-box 1.
 REGION 169 217 RGG-box 2.  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Reference proteome; Repeat; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 217 AA 
Protein Sequence
MSFRGGGRGG FNRGGGGGGF NRGGSSNHFR GGGGGGGGGN FRGGGRGGFG RGGGRGGFNK 60
GQDQGPPERV VLLGEFLHPC EDDIVCKCTT DENKVPYFNA PVYLENKEQI GKVDEIFGQL 120
RDFYFSVKLS ENMKASSFKK LQKFYIDPYK LLPLQRFLPR PPGEKGPPRG GGRGGRGGGR 180
GGGGRGGGRG GGFRGGRGGG GGGFRGGRGG GFRGRGH 217 
Gene Ontology
 GO:0031429; C:box H/ACA snoRNP complex; IEA:InterPro.
 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0005261; F:cation channel activity; TAS:ProtInc.
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0030515; F:snoRNA binding; IEA:InterPro.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
 GO:0031120; P:snRNA pseudouridine synthesis; IEA:InterPro. 
Interpro
 IPR007504; H/ACA_rnp_Gar1/Naf1.
 IPR021154; H/ACA_rnp_Gar1_euk.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF04410; Gar1 
SMART
  
PROSITE
  
PRINTS