CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011107
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCAAT/enhancer-binding protein zeta 
Protein Synonyms/Alias
 CCAAT-box-binding transcription factor; CBF; CCAAT-binding factor 
Gene Name
 CEBPZ 
Gene Synonyms/Alias
 CBF2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
98LNLAKYTKASLVEEDubiquitination[1, 2]
314FSQRPFDKLEQLSSGubiquitination[1]
390LLVQVVNKLGDPQNRubiquitination[3]
417LCKHPNMKGVVSGEVubiquitination[2]
481KKKDVESKMLSALLTubiquitination[2]
695KGGKQLNKYDPFSRNacetylation[4, 5, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Stimulates transcription from the HSP70 promoter. 
Sequence Annotation
 MOD_RES 629 629 Phosphoserine.
 MOD_RES 695 695 N6-acetyllysine.
 MOD_RES 835 835 Phosphoserine.
 MOD_RES 973 973 Phosphoserine.
 MOD_RES 978 978 Phosphoserine.  
Keyword
 Acetylation; Activator; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1054 AA 
Protein Sequence
MAAVKEPLEF HAKRPWRPEE AVEDPDEEDE DNTSEAENGF SLEEVLRLGG TKQDYLMLAT 60
LDENEEVIDG GKKGAIDDLQ QGELEAFIQN LNLAKYTKAS LVEEDEPAEK ENSSKKEVKI 120
PKINNKNTAE SQRTSVNKVK NKNRPEPHSD ENGSTTPKVK KDKQNIFEFF ERQTLLLRPG 180
GKWYDLEYSN EYSLKPQPQD VVSKYKTLAQ KLYQHEINLF KSKTNSQKGA SSTWMKAIVS 240
SGTLGDRMAA MILLIQDDAV HTLQFVETLV NLVKKKGSKQ QCLMALDTFK ELLITDLLPD 300
NRKLRIFSQR PFDKLEQLSS GNKDSRDRRL ILWYFEHQLK HLVAEFVQVL ETLSHDTLVT 360
TKTRALTVAH ELLCNKPEEE KALLVQVVNK LGDPQNRIAT KASHLLETLL CKHPNMKGVV 420
SGEVERLLFR SNISSKAQYY AICFLNQMAL SHEESELANK LITVYFCFFR TCVKKKDVES 480
KMLSALLTGV NRAYPYSQTG DDKVREQIDT LFKVLHIVNF NTSVQALMLL FQVMNSQQTI 540
SDRYYTALYR KMLDPGLMTC SKQAMFLNLV YKSLKADIVL RRVKAFVKRL LQVTCQQMPP 600
FICGALYLVS EILKAKPGLR SQLDDHPESD DEENFIDAND DEDMEKFTDA DKETEIVKKL 660
ETEETVPETD VETKKPEVAS WVHFDNLKGG KQLNKYDPFS RNPLFCGAEN TSLWELKKLS 720
VHFHPSVALF AKTILQGNYI QYSGDPLQDF TLMRFLDRFV YRNPKPHKGK ENTDSVVMQP 780
KRKHFIKDIR HLPVNSKEFL AKEESQIPVD EVFFHRYYKK VAVKEKQKRD ADEESIEDVD 840
DEEFEELIDT FEDDNCFSSG KDDMDFAGNV KKRTKGAKDN TLDEDSEGSD DELGNLDDDE 900
VSLGSMDDEE FAEVDEDGGT FMDVLDDESE SVPELEVHSK VSTKKSKRKG TDDFDFAGSF 960
QGPRKKKRNL NDSSLFVSAE EFGHLLDENM GSKFDNIGMN AMANKDNASL KQLRWEAERD 1020
DWLHNRDAKS IIKKKKHFKK KRIKTTQKTK KQRK 1054 
Gene Ontology
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR016024; ARM-type_fold.
 IPR005612; CCAAT-binding_factor. 
Pfam
 PF03914; CBF 
SMART
  
PROSITE
  
PRINTS