CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000405
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NF-kappa-B-repressing factor 
Protein Synonyms/Alias
 NFkB-repressing factor; Protein ITBA4; Transcription factor NRF 
Gene Name
 NKRF 
Gene Synonyms/Alias
 ITBA4; NRF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68HFVASSSKDERQEDPubiquitination[1]
196EMTSGSDKINYTYMLubiquitination[1]
211TRCIQACKTNPEYIYubiquitination[1]
234ADIPKNKKLLTDGYAubiquitination[1]
259TTGYAGSKNGSRDRAubiquitination[1, 2]
407AAADEALKILQKTQPubiquitination[1]
411EALKILQKTQPTYPSubiquitination[1]
524TVINNLKKGAVEDVIubiquitination[1]
571WTGGGLGKSGEGIREubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. 
Sequence Annotation
 DOMAIN 551 596 G-patch.
 DOMAIN 600 664 R3H.
 DNA_BIND 296 388
 REGION 1 296 Active repression domain.
 MOTIF 25 45 Nuclear localization signal.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 690 AA 
Protein Sequence
MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK FHARPRFEPV 60
HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY TQDSFSIQDG NSQYCDSSGF 120
ILTKDQPVTA NMYFDSGNPA PSTTSQQANS QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT 180
IWKNLSNPEM TSGSDKINYT YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY 240
ACEVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI 300
GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI GILNNSASFN 360
KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA AADEALKILQ KTQPTYPSVK 420
SSQCHTGSSP RGSGKKKDIK DLVVYENSSN PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC 480
KVILESEVIA EAVGVKKTVK YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS 540
AEEAYKQQIK EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK 600
IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH GVGHDRYLVV 660
GRKRRKEDLL DQLKQEGQVG HYELVMPQAN 690 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR000467; G_patch_dom.
 IPR001374; R3H_ss-bd. 
Pfam
 PF01585; G-patch
 PF01424; R3H 
SMART
 SM00358; DSRM
 SM00443; G_patch
 SM00393; R3H 
PROSITE
 PS50174; G_PATCH
 PS51061; R3H 
PRINTS