CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002021
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Metallothionein-2 
Protein Synonyms/Alias
 MT-2; Metallothionein-2A; Metallothionein-II; MT-II 
Gene Name
 MT2A 
Gene Synonyms/Alias
 CES1; MT2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51CAQGCICKGASDKCSacetylation[1, 2]
51CAQGCICKGASDKCSubiquitination[3, 4]
Reference
 [1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. 
Sequence Annotation
 REGION 1 29 Beta.
 REGION 30 61 Alpha.
 METAL 5 5 Divalent metal cation; cluster B.
 METAL 7 7 Divalent metal cation; cluster B.
 METAL 13 13 Divalent metal cation; cluster B.
 METAL 15 15 Divalent metal cation; cluster B.
 METAL 19 19 Divalent metal cation; cluster B.
 METAL 21 21 Divalent metal cation; cluster B.
 METAL 24 24 Divalent metal cation; cluster B.
 METAL 26 26 Divalent metal cation; cluster B.
 METAL 29 29 Divalent metal cation; cluster B.
 METAL 33 33 Divalent metal cation; cluster A.
 METAL 34 34 Divalent metal cation; cluster A.
 METAL 36 36 Divalent metal cation; cluster A.
 METAL 37 37 Divalent metal cation; cluster A.
 METAL 41 41 Divalent metal cation; cluster A.
 METAL 44 44 Divalent metal cation; cluster A.
 METAL 48 48 Divalent metal cation; cluster A.
 METAL 50 50 Divalent metal cation; cluster A.
 METAL 57 57 Divalent metal cation; cluster A.
 METAL 59 59 Divalent metal cation; cluster A.
 METAL 60 60 Divalent metal cation; cluster A.
 MOD_RES 1 1 N-acetylmethionine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Metal-binding; Metal-thiolate cluster; Polymorphism; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 61 AA 
Protein Sequence
MDPNCSCAAG DSCTCAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCIC KGASDKCSCC 60
A 61 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO:0008144; F:drug binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0006878; P:cellular copper ion homeostasis; TAS:ProtInc.
 GO:0035690; P:cellular response to drug; IDA:UniProtKB.
 GO:0036018; P:cellular response to erythropoietin; IEP:UniProtKB.
 GO:0036016; P:cellular response to interleukin-3; IEP:UniProtKB.
 GO:0071294; P:cellular response to zinc ion; IEP:UniProtKB.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0045926; P:negative regulation of growth; ISS:UniProtKB. 
Interpro
 IPR017854; Metalthion_dom.
 IPR023587; Metalthion_dom_vert.
 IPR003019; Metalthion_sfam_euk.
 IPR000006; Metalthion_vert.
 IPR018064; Metalthion_vert_metal_BS. 
Pfam
 PF00131; Metallothio 
SMART
  
PROSITE
 PS00203; METALLOTHIONEIN_VRT 
PRINTS
 PR00860; MTVERTEBRATE.