UniProt Accession

 RIBD_ECOLI; P25539; Q2MC12 

Genbank Protein ID

 X64395; U82664; U00096; AP009048 

Genbank Nucleotide ID

 CAA45735.1; AAB40170.1; AAC73517.1; BAE76194.1 

Protein Name

 Riboflavin biosynthesis protein RibD 

Protein Synonyms/Alias

 Diaminohydroxyphosphoribosylaminopyrimidine deaminase; DRAP deaminase; Riboflavin-specific deaminase; 5-amino-6-(5-phosphoribosylamino)uracil reductase; HTP reductase 

Gene Name

 ribD 

Gene Synonyms/Alias

 ribG; ybaE; b0414; JW0404 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
276	TLLIPEHKGHLDLVV	acetylation	[1]
341	CTLPGLEKLADAPQF	acetylation	[1]
349	LADAPQFKFKEIRHV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate. 

Sequence Annotation

 NP_BIND 161 164 NADP.
 NP_BIND 301 304 NADP.
 REGION 1 145 Deaminase.
 REGION 146 367 Reductase.
 ACT_SITE 52 52 Proton donor (By similarity).
 METAL 50 50 Zinc; catalytic (By similarity).
 METAL 75 75 Zinc; catalytic (By similarity).
 METAL 84 84 Zinc; catalytic (By similarity).
 BINDING 168 168 Substrate.
 BINDING 170 170 NADP.
 BINDING 184 184 Substrate.
 BINDING 196 196 NADP.
 BINDING 200 200 NADP.
 BINDING 204 204 Substrate; via amide nitrogen.
 BINDING 207 207 Substrate.
 BINDING 234 234 NADP.
 BINDING 299 299 Substrate.  

Keyword

 3D-structure; Complete proteome; Hydrolase; Metal-binding; Multifunctional enzyme; NADP; Oxidoreductase; Reference proteome; Riboflavin biosynthesis; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 367 AA 

Protein Sequence

Gene Ontology

 GO:0008703; F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity; IDA:EcoCyc.
 GO:0008835; F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity; IDA:EcoCyc.
 GO:0050661; F:NADP binding; IDA:EcoCyc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0009231; P:riboflavin biosynthetic process; IDA:EcoCyc. 

Interpro

 IPR016192; APOBEC/CMP_deaminase_Zn-bd.
 IPR002125; CMP_dCMP_Zn-bd.
 IPR016193; Cytidine_deaminase-like.
 IPR024072; DHFR-like_dom.
 IPR004794; Eubact_RibD.
 IPR011549; RibD_C.
 IPR002734; RibDG_C. 

Pfam

 PF00383; dCMP_cyt_deam_1
 PF01872; RibD_C 

SMART

  

PROSITE

 PS00903; CYT_DCMP_DEAMINASES 

PRINTS