| Tag | Content |
|---|
| CPLM ID | CPLM-011313 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | ERAD-associated E3 ubiquitin-protein ligase component HRD3 |
Protein Synonyms/Alias | HMG-CoA reductase degradation protein 3 |
Gene Name | HRD3 |
Gene Synonyms/Alias | YLR207W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 188 | AAQLGNLKAKQVLAY | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Component of the endoplasmic reticulum quality control (ERQC) system involved in ubiquitin-dependent degradation of missfolded endoplasmic reticulum proteins. Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD- M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome. ERAD-M substrates are processed by the same HRD1-HRD3 core complex, but only a subset of the other components is required for ERAD-M. Stabilizes the HRD1 ubiquitin-protein ligase. Has also a function in recruiting misfolded protein substrates. |
Sequence Annotation | REPEAT 103 139 Sel1-like 1.
REPEAT 143 186 Sel1-like 2.
REPEAT 187 222 Sel1-like 3.
REPEAT 413 445 Sel1-like 4.
REPEAT 552 595 Sel1-like 5.
REPEAT 596 627 Sel1-like 6.
REPEAT 628 663 Sel1-like 7.
CARBOHYD 101 101 N-linked (GlcNAc...) (Potential).
CARBOHYD 123 123 N-linked (GlcNAc...) (Potential).
CARBOHYD 142 142 N-linked (GlcNAc...) (Potential).
CARBOHYD 429 429 N-linked (GlcNAc...) (Potential).
CARBOHYD 611 611 N-linked (GlcNAc...) (Potential). |
Keyword | Complete proteome; Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 833 AA |
Protein Sequence | MITLLLYLCV ICNAIVLIRA DSIADPWPEA RHLLNTIAKS RDPMKEAAME PNADEFVGFY 60
VPMDYSPRNE EKNYQSIWQN EITDSQRHIY ELLVQSSEQF NNSEATYTLS QIHLWSQYNF 120
PHNMTLAHKY LEKFNDLTHF TNHSAIFDLA VMYATGGCAS GNDQTVIPQD SAKALLYYQR 180
AAQLGNLKAK QVLAYKYYSG FNVPRNFHKS LVLYRDIAEQ LRKSYSRDEW DIVFPYWESY 240
NVRISDFESG LLGKGLNSVP SSTVRKRTTR PDIGSPFIAQ VNGVQMTLQI EPMGRFAFNG 300
NDGNINGDED DEDASERRII RIYYAALNDY KGTYSQSRNC ERAKNLLELT YKEFQPHVDN 360
LDPLQVFYYV RCLQLLGHMY FTGEGSSKPN IHMAEEILTT SLEISRRAQG PIGRACIDLG 420
LINQYITNNI SQAISYYMKA MKTQANNGIV EFQLSKLATS FPEEKIGDPF NLMETAYLNG 480
FIPAIYEFAV MIESGMNSKS SVENTAYLFK TFVDKNEAIM APKLRTAFAA LINDRSEVAL 540
WAYSQLAEQG YETAQVSAAY LMYQLPYEFE DPPRTTDQRK TLAISYYTRA FKQGNIDAGV 600
VAGDIYFQMQ NYSKAMALYQ GAALKYSIQA IWNLGYMHEH GLGVNRDFHL AKRYYDQVSE 660
HDHRFYLASK LSVLKLHLKS WLTWITREKV NYWKPSSPLN PNEDTQHSKT SWYKQLTKIL 720
QRMRHKEDSD KAAEDSHKHR TVVQNGANHR GDDQEEASEI LGFQMEDLVT MGCILGIFLL 780
SILMSTLAAR RGWNVRFNGA QLNANGNRQQ EQQQQQQAQG PPGWDFNVQI FAI 833 |
Gene Ontology | GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IDA:SGD. GO:0000838; C:Hrd1p ubiquitin ligase ERAD-M complex; IDA:SGD. GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0034099; C:luminal surveillance complex; IDA:SGD. GO:0030433; P:ER-associated protein catabolic process; IDA:SGD. GO:0016567; P:protein ubiquitination; IDA:GOC. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |