CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015526
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCR4-NOT transcription complex subunit 1 
Protein Synonyms/Alias
 CCR4-associated factor 1 
Gene Name
 Cnot1 
Gene Synonyms/Alias
 Kiaa1007 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
231APLLYPEKRDILMDRubiquitination[1]
355LNPSLNFKEVTYELDubiquitination[1]
808NDPFVQRKLGTSGLNubiquitination[1]
1708YGSPWCNKQITRCLIubiquitination[1]
2178VMPPQFKKDLDSYLKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA- mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of emryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages. 
Sequence Annotation
 REGION 1089 1604 Interaction with CNOT6, CNOT6L, CNOT7 and
 MOTIF 153 157 LXXLL.
 MOTIF 181 185 LXXLL.
 MOTIF 223 227 LXXLL.
 MOTIF 570 574 LXXLL.
 MOTIF 1638 1642 LXXLL.
 MOTIF 1941 1945 LXXLL.
 MOTIF 2095 2099 LXXLL.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Nucleus; Reference proteome; Repressor; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2375 AA 
Protein Sequence
MNLDSLSLAL SQISYLVDNL TKKNYRASQQ EIQHIVNRHG PEADRHLLRC LFSHVDFSGD 60
GKSSGKDFHQ TQFLIQECAS LITKPNFIST LSYAIDNPLH YQKSLKPAPH LFAQLSKVLK 120
LSKVQEVIFG LALLNSSSPD LRGFAAQFIK QKLPDLLRSY IDADVSGNQE GGFQDIAIEV 180
LHLLLSHLLF GQKGAFGVGQ EQIDAFLKTL RRDFPQERCP VVLAPLLYPE KRDILMDRIL 240
PDSGGVAKTM MESSLADFMQ EVGYGFCASI EECRNIIMQF GVREVTAAQV ARVLGMMART 300
HSGLTDGIPL QSISAPGSGI WSDGKDKSEG AQAHTWNVEV LIDVLKELNP SLNFKEVTYE 360
LDHPGFQIRD SKGLHNVVYG IQRGLGMEVF PVDFIYRPWK HAEGQLSFIQ HSLINPEVFC 420
FADYPCHTVA TDILKAPPED DNREIATWKS LDLIESLLRL AEVGQYEQVK QLFSFPIKHC 480
PDMLVLALLQ INTSWHTLRH ELISTLMPIF LGNHPNSAII LHYAWHGQGQ SPSIRQLIMH 540
AMAEWYMRGE QYDQAKLSRI LDVAQDLKAL SMLLNGTPFA FVIDLAALAS RREYLKLDKW 600
LTDKIREHGE PFIQACMTFL KRRCPSILGG LAPEKDQPKS AQLPAETLAT MLACLQACAG 660
SVSQELSETI LTMVANCSNV MNKARQPPPG VMPKGRPPSA SSLDAISPVQ IDPLAGMASL 720
SIGGSAAPHT QSMQGFPPNL GSAFSTPQSP AKAFPPLSTP NQTTAFSGIG GLSSQLPVGG 780
LGTGSLTGIG TGALGLPAVN NDPFVQRKLG TSGLNQPTFQ QSKMKPSDLS QVWPEANQHF 840
SKEIDDEANS YFQRIYNHPP HPTMSVDEVL EMLQRFKDST IKREREVFNC MLRNLFEEYR 900
FFPQYPDKEL HITACLFGGI IEKGLVTYMA LGLALRYVLE ALRKPFGSKM YYFGIAALDR 960
FKNRLKDYPQ YCQHLASISH FMQFPHHLQE YIEYGQQSRD PPVKMQGSIT TPGSIALAQA 1020
QAQAQVPAKA PLAGQVNTMV TTSTTTTVAK TVTVTKPTGV SFKKDVPPSI NTTNIDTLLV 1080
ATDQTERIVE PPENIQEKIA FIFNNLSQSN MTQKVEELKE TVKEEFMPWV SQYLVMKRVS 1140
IEPNFHSLYS NFLDTLKNPE FNKMVLNETY RNIKVLLTSD KAAANFSDRS LLKNLGHWLG 1200
MITLAKNKPI LHTDLDVKSL LLEAYVKGQQ ELLYVVPFVA KVLESSIRSL VFRPPNPWTM 1260
AIMNVLAELH QEHDLKLNLK FEIEVLCKNL ALDINELKPG NLLKDKDRLK NLDEQLSAPK 1320
KDVKQPEELP AITTTTTSTT PATSTTCTAT VPPQPQYSYH DINVYSLAGL APHITLNPTI 1380
PLFQAHPQLK QCVRQAIERA VQELVHPVVD RSIKIAMTTC EQIVRKDFAL DSEESRMRIA 1440
AHHMMRNLTA GMAMITCREP LLMSISTNLK NSFASALRTA SPQQREMMDQ AAAQLAQDNC 1500
ELACCFIQKT AVEKAGPEMD KRLATEFELR KHARQEGRRY CDPVVLTYQA ERMPEQIRLK 1560
VGGVDPKQLA VYEEFARNVP GFLPTNDLSQ PTGFLAQPMK QAWATDDVAQ IYDKCITELE 1620
QHLHAIPPTL AMNPQAQALR SLLEVVVLSR NSRDAIAALG LLQKAVEGLL DATSGADADL 1680
LLRYRECHLL VLKALQDGRA YGSPWCNKQI TRCLIECRDE YKYNVEAVEL LIRNHLVNMQ 1740
QYDLHLAQSM ENGLNYMAVA FAMQLVKILL VDERSVAHIT EADLFHTIET LMRINAHSRG 1800
NAPEGLPQLM EVVRSNYEAM IDRAHGGPNF MMHSGISQAS EYDDPPGLRE KAEYLLREWV 1860
NLYHSAAAGR DSTKAFSAFV GQMHQQGILK TDDLITRFFR LCTEMCVEIS YRAQAEQQHN 1920
PAANPTMIRA KCYHNLDAFV RLIALLVKHS GEATNTVTKI NLLNKVLGIV VGVLLQDHDV 1980
RQSEFQQLPY HRIFIMLLLE LNAPEHVLET INFQTLTAFC NTFHILRPTK APGFVYAWLE 2040
LISHRIFIAR MLAHTPQQKG WPMYAQLLID LFKYLAPFLR NVELTKPMQI LYKGTLRVLL 2100
VLLHDFPEFL CDYHYGFCDV IPPNCIQLRN LILSAFPRNM RLPDPFTPNL KVDMLSEINI 2160
APRILTNFTG VMPPQFKKDL DSYLKTRSPV TFLSDLRSNL QVSNEPGNRY NLQLINALVL 2220
YVGTQAIAHI HNKGSTPSMS TITHSAHMDI FQNLAVDLDT EGRYLFLNAI ANQLRYPNSH 2280
THYFSCTMLY LFAEANTEAI QEQITRVLLE RLIVNRPHPW GLLITFIELI KNPAFKFWNH 2340
EFVHCAPEIE KLFQSVAQCC MGQKQAQQVM EGTGAS 2376 
Gene Ontology
 GO:0030014; C:CCR4-NOT complex; ISS:UniProtKB.
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0030331; F:estrogen receptor binding; ISS:UniProtKB.
 GO:0042974; F:retinoic acid receptor binding; ISS:UniProtKB.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; ISS:UniProtKB.
 GO:0048387; P:negative regulation of retinoic acid receptor signaling pathway; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; ISS:UniProtKB.
 GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; ISS:UniProtKB.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISS:UniProtKB.
 GO:2000036; P:regulation of stem cell maintenance; IMP:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0001829; P:trophectodermal cell differentiation; IMP:UniProtKB. 
Interpro
 IPR007196; CCR4-Not_Not1_C.
 IPR024557; CCR4-Not_Not1su_DUF3819. 
Pfam
 PF12842; DUF3819
 PF04054; Not1 
SMART
  
PROSITE
  
PRINTS