| Tag | Content |
|---|
| CPLM ID | CPLM-016750 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin-like modifier-activating enzyme 6 |
Protein Synonyms/Alias | Ubiquitin-activating enzyme 6; Ubiquitin-activating enzyme E1-like protein 2; E1-L2 |
Gene Name | Uba6 |
Gene Synonyms/Alias | Ube1l2 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 503 | TDPDLIEKSNLNRQF | ubiquitination | [1] | | 544 | KIDAHLNKVCPATES | ubiquitination | [1] | | 652 | FESSFSHKPSLFNKF | ubiquitination | [1] | | 739 | SLFWQSPKRPPSPIK | ubiquitination | [1] | | 836 | EEALSSNKATKSDLQ | ubiquitination | [1] | | 839 | LSSNKATKSDLQMTV | ubiquitination | [1] | | 882 | IEPADRFKTKRIAGK | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34 (By similarity). Essential for embryonic development. Required for UBD/FAT10 conjugation. |
Sequence Annotation | NP_BIND 470 500 ATP (By similarity).
ACT_SITE 625 625 Glycyl thioester intermediate.
MOD_RES 1 1 N-acetylmethionine (By similarity).
MOD_RES 544 544 N6-acetyllysine (By similarity). |
Keyword | Acetylation; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1053 AA |
Protein Sequence | MERSEPLAVL SCEEASCSSW GACGASKNLP TMTTESLEID DGLYSRQRYV LGDTAMQKMA 60
KSCVFLSGMG GLGVEIAKNL VLAGIKALTI HDTKKCQAWD LGTNFFLCED DVVNERNRAE 120
AVLHRIAELN PYVQVSSSSA PLDETTDLSF LEKYQCVVLT EIKLTLQKKI NNFCHSHCPP 180
IKFISADVHG IWSRLFCDFG DEFEVSDTTG EEPKEIFISN ITQANPGIVT CLESHPHKLE 240
TGQFLTFREI HGMTGLNGSV QQITVISPFS FSIGDTTKLD PYLHGGIAVQ VKTPKTFCFE 300
PLESQIKHPR CLIADFSKPE APLEIHLAML ALDQFQENYN RKPNIRCQQD SDELLKLTVS 360
INETLEEKPE VNADIVHWLS WTAQGFLPPL AAAVGGVASQ EVLKAVTGKF SPLCQWLYLE 420
AADTVESLGN PGHEEFLPRG DRYDAIRACI GNTLCQKLQN LNIFLVGCGA IGCEMLKNFA 480
LLGVGTGREK GMVTVTDPDL IEKSNLNRQF LFRPHHIQKP KSYTAAEATL KINPQLKIDA 540
HLNKVCPATE SIYSDEFYTK QDIIITALDN VEARRYVDSR CLANLRPLLD SGTMGTKGHT 600
EIIVPQLTES YNSHRDPPEE EIPFCTLKSF PAAIEHTIQW ARDKFESSFS HKPSLFNKFW 660
QAYPSAEDVL QKIQNGQSLE GCFQVIKLLS RRPRIWSQCV ELARLKFEKY FNHKALQLLH 720
CFPLETRLKD GSLFWQSPKR PPSPIKFDLN EPLHLSFLQS AAKLYATVYC IPFSEKDLSV 780
NSLMDILSEV KIEEFKPSNK VVQTDETARK PDHVPVSSED ERNAVFQLEE ALSSNKATKS 840
DLQMTVLSFE KDDDRNGHID FITAASNLRA KMYSIEPADR FKTKRIAGKI IPAIATSTAA 900
VSGLVALEMI KVAGGYPFDA YKNCFLNLAI PIIVFTETSE VRKTEIRNGI SFTIWDRWTV 960
HGKEDFTLSD FINAVKENYG IEPTMVVQGV KMLYVPVMPG HAKRLKLTMH KLVKPSTEKK 1020
YVDLTVSFAP DADGDEDLPG PPVRYYFSHD TNE 1053 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:Compara. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0019780; F:FAT10 activating enzyme activity; IMP:UniProtKB. GO:0016874; F:ligase activity; IEA:UniProtKB-KW. GO:0016567; P:protein ubiquitination; IMP:UniProtKB. GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |