CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017977
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 15 
Protein Synonyms/Alias
 GTPase-activating protein RAB7; GAP for RAB7; Rab7-GAP 
Gene Name
 TBC1D15 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43GILRVLEKDAEVIVDubiquitination[1]
68SSILYARKDSSSVVEubiquitination[1]
81VEWTQAPKERGHRGSubiquitination[1]
107MVNTVSFKRKPHTNGubiquitination[1]
190LCESPQDKRTLLVNCubiquitination[2]
200LLVNCQNKSLSQSFEubiquitination[2]
220PAYGLIQKIKKDPYTubiquitination[1, 2]
222YGLIQKIKKDPYTATubiquitination[1, 2]
223GLIQKIKKDPYTATMubiquitination[1, 2]
235ATMIGFSKVTNYIFDubiquitination[1]
271SDAIPGLKINQQEEPubiquitination[1]
307VSLEEWTKNIDSEGRubiquitination[1]
322ILNVDNMKQMIFRGGubiquitination[1]
352YFPWDSTKEERTQLQubiquitination[1]
396DYRSLIEKDVNRTDRubiquitination[1]
572YGFNEILKHINELSMubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6 (By similarity). 
Sequence Annotation
 DOMAIN 346 556 Rab-GAP TBC.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 70 70 Phosphoserine.
 MOD_RES 180 180 Phosphotyrosine (By similarity).
 MOD_RES 205 205 Phosphoserine (By similarity).
 MOD_RES 675 675 Phosphoserine.
 MOD_RES 689 689 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 691 AA 
Protein Sequence
MAAAGVVSGK IIYEQEGVYI HSSCGKTNDQ DGLISGILRV LEKDAEVIVD WRPLDDALDS 60
SSILYARKDS SSVVEWTQAP KERGHRGSEH LNSYEAEWDM VNTVSFKRKP HTNGDAPSHR 120
NGKSKWSFLF SLTDLKSIKQ NKEGMGWSYL VFCLKDDVVL PALHFHQGDS KLLIESLEKY 180
VVLCESPQDK RTLLVNCQNK SLSQSFENLL DEPAYGLIQK IKKDPYTATM IGFSKVTNYI 240
FDSLRGSDPS THQRPPSEMA DFLSDAIPGL KINQQEEPGF EVITRIDLGE RPVVQRREPV 300
SLEEWTKNID SEGRILNVDN MKQMIFRGGL SHALRKQAWK FLLGYFPWDS TKEERTQLQK 360
QKTDEYFRMK LQWKSISQEQ EKRNSRLRDY RSLIEKDVNR TDRTNKFYEG QDNPGLILLH 420
DILMTYCMYD FDLGYVQGMS DLLSPLLYVM ENEVDAFWCF ASYMDQMHQN FEEQMQGMKT 480
QLIQLSTLLR LLDSGFCSYL ESQDSGYLYF CFRWLLIRFK REFSFLDILR LWEVMWTELP 540
CTNFHLLLCC AILESEKQQI MEKHYGFNEI LKHINELSMK IDVEDILCKA EAISLQMVKC 600
KELPQAVCEI LGLQGSEVTT PDSDVGEDEN VVMTPCPTSA FQSNALPTLS ASGARNDSPT 660
QIPVSSDVCR LTPA 674 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005576; C:extracellular region; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005097; F:Rab GTPase activator activity; ISS:UniProtKB. 
Interpro
 IPR021935; DUF3548.
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF12068; DUF3548
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS