CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018897
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone acetyltransferase KAT5 
Protein Synonyms/Alias
 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein 
Gene Name
 KAT5 
Gene Synonyms/Alias
 HTATIP; TIP60 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
263DDIVTRMKNIECIELubiquitination[1]
307LKYGRSLKCLQRHLTubiquitination[1]
315CLQRHLTKCDLRHPPubiquitination[1]
343EIDGRKNKSYSQNLCubiquitination[1]
437GKTGTPEKPLSDLGLubiquitination[1]
531RLLRIDSKCLHFTPKubiquitination[1]
538KCLHFTPKDWSKRGKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. 
Sequence Annotation
 ZN_FING 261 283 C2HC-type.
 REGION 377 383 Acetyl-CoA binding.
 ACT_SITE 327 327 By similarity.
 ACT_SITE 369 369 Nucleophile (By similarity).
 BINDING 372 372 Acetyl-CoA.
 BINDING 407 407 Acetyl-CoA.
 MOD_RES 52 52 N6-acetyllysine.
 MOD_RES 86 86 Phosphoserine.
 MOD_RES 90 90 Phosphoserine; by CDK1.
 MOD_RES 327 327 N6-acetyllysine; by autocatalysis (By
 CROSSLNK 430 430 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 451 451 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Activator; Acyltransferase; Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; Direct protein sequencing; Growth regulation; Host-virus interaction; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Transferase; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MAEVVSPVPG AGRREPGEVG RARGPPVADP GVALSPQGEI IEGCRLPVLR RNQDNEDEWP 60
LAEILSVKDI SGRKLFYVHY IDFNKRLDEW VTHERLDLKK IQFPKKEAKT PTKNGLPGSR 120
PGSPEREVPA SAQASGKTLP IPVQITLRFN LPKEREAIPG GEPDQPLSSS SCLQPNHRST 180
KRKVEVVSPA TPVPSETAPA SVFPQNGAAR RAVAAQPGRK RKSNCLGTDE DSQDSSDGIP 240
SAPRMTGSLV SDRSHDDIVT RMKNIECIEL GRHRLKPWYF SPYPQELTTL PVLYLCEFCL 300
KYGRSLKCLQ RHLTKCDLRH PPGNEIYRKG TISFFEIDGR KNKSYSQNLC LLAKCFLDHK 360
TLYYDTDPFL FYVMTEYDCK GFHIVGYFSK EKESTEDYNV ACILTLPPYQ RRGYGKLLIE 420
FSYELSKVEG KTGTPEKPLS DLGLLSYRSY WSQTILEILM GLKSESGERP QITINEISEI 480
TSIKKEDVIS TLQYLNLINY YKGQYILTLS EDIVDGHERA MLKRLLRIDS KCLHFTPKDW 540
SKRGKW 546 
Gene Ontology
 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0032777; C:Piccolo NuA4 histone acetyltransferase complex; IDA:UniProtKB.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0006978; P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; IDA:UniProtKB.
 GO:0006302; P:double-strand break repair; IMP:UniProtKB.
 GO:0016573; P:histone acetylation; IDA:UniProtKB.
 GO:0032703; P:negative regulation of interleukin-2 production; IDA:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR002717; MOZ_SAS.
 IPR025995; Tudor-knot. 
Pfam
 PF01853; MOZ_SAS
 PF11717; Tudor-knot 
SMART
 SM00298; CHROMO 
PROSITE
  
PRINTS