CPLM-024361

Genbank Nucleotide ID

RNA polymerase-associated protein RTF1 homolog

Protein Synonyms/Alias

Mus musculus (Mouse)

Position	Peptide	Type	References
48	SRGTTMVKKRKGRVV	acetylation	[1]
418	TGVVETAKVYQLGGT	acetylation	[2, 3]

[1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Binds single-stranded DNA (By similarity). Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.

DOMAIN 358 489 Plus3.
MOD_RES 58 58 Phosphoserine (By similarity).
MOD_RES 60 60 Phosphothreonine (By similarity).
MOD_RES 702 702 Phosphoserine (By similarity).

Activator; Coiled coil; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Wnt signaling pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016593; C:Cdc73/Paf1 complex; ISS:UniProtKB.
GO:0005730; C:nucleolus; IDA:MGI.
GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
GO:0001711; P:endodermal cell fate commitment; IMP:UniProtKB.
GO:0080182; P:histone H3-K4 trimethylation; IMP:UniProtKB.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
GO:0051571; P:positive regulation of histone H3-K4 methylation; IMP:UniProtKB.
GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; ISS:UniProtKB.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
GO:0019827; P:stem cell maintenance; IMP:UniProtKB.
GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.

IPR004343; Plus-3.
IPR018144; Plus3-dom_subgr.