UniProt Accession

 MCCB_MOUSE; Q3ULD5; Q3UPS6 

Genbank Protein ID

 AK132265; AK143233; AK145564; AK146865; AK146844 

Genbank Nucleotide ID

 BAE21068.1; BAE25319.1; BAE26513.1; BAE27489.1; BAE27475.1 

Protein Name

 Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial 

Protein Synonyms/Alias

 MCCase subunit beta; 3-methylcrotonyl-CoA carboxylase 2; 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta 

Gene Name

 Mccc2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
50	QENYEQMKALVSQLH	acetylation	[1, 2, 3]
70	VRLGGSEKARARHTS	acetylation	[2, 3, 4, 5]
70	VRLGGSEKARARHTS	succinylation	[5]
141	VANDATVKGGTYYPV	acetylation	[5]
141	VANDATVKGGTYYPV	succinylation	[5]
433	VAAVACAKVPKITVI	acetylation	[5]
433	VAAVACAKVPKITVI	succinylation	[5]
495	DQKAREGKQFSSAEE	acetylation	[2, 3, 4, 5]
495	DQKAREGKQFSSAEE	succinylation	[5]
506	SAEEAALKEPIIKRF	acetylation	[2]
511	ALKEPIIKRFEEEGN	acetylation	[2, 4]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism (By similarity). 

Sequence Annotation

 DOMAIN 55 557 Carboxyltransferase.
 REGION 343 372 Acyl-CoA binding (Potential).  

Keyword

 ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 563 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004485; F:methylcrotonoyl-CoA carboxylase activity; ISS:UniProtKB.
 GO:0015936; P:coenzyme A metabolic process; IEA:Compara.
 GO:0006552; P:leucine catabolic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR000022; Carboxyl_trans.
 IPR011763; COA_CT_C.
 IPR011762; COA_CT_N. 

Pfam

 PF01039; Carboxyl_trans 

SMART

  

PROSITE

 PS50989; COA_CT_CTER
 PS50980; COA_CT_NTER 

PRINTS