UniProt Accession

 NDUA4_MOUSE; Q62425; Q9CQP6 

Genbank Protein ID

 AK005084; AK008357; AK008506; AK076036; BC011114; U59509 

Genbank Nucleotide ID

 BAB25627.1; BAB25706.1; BAC36137.1; AAH11114.1; AAB03395.1 

Protein Name

 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 

Protein Synonyms/Alias

 Complex I-MLRQ; CI-MLRQ; NADH-ubiquinone oxidoreductase MLRQ subunit 

Gene Name

 Ndufa4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	RQILGQAKKHPSLIP	acetylation	[1, 2]
56	NNPEPWNKLGPNEQY	acetylation	[3]
56	NNPEPWNKLGPNEQY	ubiquitination	[4]
64	LGPNEQYKFYSVNVD	acetylation	[5]
64	LGPNEQYKFYSVNVD	ubiquitination	[4]
74	SVNVDYSKLKKEGPD	acetylation	[2]
74	SVNVDYSKLKKEGPD	ubiquitination	[4]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). 

Sequence Annotation

 MOD_RES 56 56 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Respiratory chain; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 82 AA 

Protein Sequence

Gene Ontology

 GO:0005751; C:mitochondrial respiratory chain complex IV; IDA:UniProtKB.
 GO:0004129; F:cytochrome-c oxidase activity; IEA:Compara.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 

Interpro

 IPR010530; B12D. 

Pfam

 PF06522; B12D 

SMART

  

PROSITE

  

PRINTS