CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019580
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting nexin-18 
Protein Synonyms/Alias
 SH3 and PX domain-containing protein 3B; Sorting nexin-associated Golgi protein 1 
Gene Name
 SNX18 
Gene Synonyms/Alias
 SH3PXD3B; SNAG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
314VPVHRRYKHFDWLYAubiquitination[1]
326LYARLAEKFPVISVPubiquitination[1]
338SVPHLPEKQATGRFEubiquitination[1]
432ESKIDGFKCFTKKMDubiquitination[1]
437GFKCFTKKMDDSALQubiquitination[1]
466GFKKEYQKVGQSFRGubiquitination[1]
562EGKMEVQKADGIQDRubiquitination[1, 2]
613FFQKVTQKLEEALHKubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane. 
Sequence Annotation
 DOMAIN 1 61 SH3.
 DOMAIN 276 386 PX.
 DOMAIN 421 628 BAR.
 BINDING 312 312 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 314 314 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 338 338 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 352 352 Phosphatidylinositol 4,5-bisphosphate (By  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Endocytosis; Endosome; Lipid-binding; Membrane; Polymorphism; Protein transport; Reference proteome; SH3 domain; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 628 AA 
Protein Sequence
MALRARALYD FRSENPGEIS LREHEVLSLC SEQDIEGWLE GVNSRGDRGL FPASYVQVIR 60
APEPGPAGDG GPGAPARYAN VPPGGFEPLP VAPPASFKPP PDAFQALLQP QQAPPPSTFQ 120
PPGAGFPYGG GALQPSPQQL YGGYQASQGS DDDWDDEWDD SSTVADEPGA LGSGAYPDLD 180
GSSSAGVGAA GRYRLSTRSD LSLGSRGGSV PPQHHPSGPK SSATVSRNLN RFSTFVKSGG 240
EAFVLGEASG FVKDGDKLCV VLGPYGPEWQ ENPYPFQCTI DDPTKQTKFK GMKSYISYKL 300
VPTHTQVPVH RRYKHFDWLY ARLAEKFPVI SVPHLPEKQA TGRFEEDFIS KRRKGLIWWM 360
NHMASHPVLA QCDVFQHFLT CPSSTDEKAW KQGKRKAEKD EMVGANFFLT LSTPPAAALD 420
LQEVESKIDG FKCFTKKMDD SALQLNHTAN EFARKQVTGF KKEYQKVGQS FRGLSQAFEL 480
DQQAFSVGLN QAIAFTGDAY DAIGELFAEQ PRQDLDPVMD LLALYQGHLA NFPDIIHVQK 540
GALTKVKESR RHVEEGKMEV QKADGIQDRC NTISFATLAE IHHFHQIRVR DFKSQMQHFL 600
QQQIIFFQKV TQKLEEALHK YDSV 624 
Gene Ontology
 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:UniProtKB.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006897; P:endocytosis; IDA:UniProtKB.
 GO:0043547; P:positive regulation of GTPase activity; IDA:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR001683; Phox.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR014536; Snx9.
 IPR019497; Sorting_nexin_WASP-bd-dom. 
Pfam
 PF10456; BAR_3_WASP_bdg
 PF00787; PX
 PF07653; SH3_2 
SMART
 SM00312; PX
 SM00326; SH3 
PROSITE
 PS51021; BAR
 PS50195; PX
 PS50002; SH3 
PRINTS