UniProt Accession

 P5CS_MOUSE; Q9Z110; Q8BGM2; Q9R1P6 

Genbank Protein ID

 AF056573; AF056574; AK151072; AK153946; AK168905; CH466534; BC033427; BC037699 

Genbank Nucleotide ID

 AAD17517.1; AAD17518.1; BAE30088.1; BAE32270.1; BAE40719.1; EDL41837.1; AAH33427.1; AAH37699.1 

Protein Name

 Delta-1-pyrroline-5-carboxylate synthase 

Protein Synonyms/Alias

 P5CS; Aldehyde dehydrogenase family 18 member A1; Glutamate 5-kinase; GK; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase 

Gene Name

 Aldh18a1 

Gene Synonyms/Alias

 P5cs; Pycs 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
311	GLGGMEAKVKAALWA	acetylation	[1]
311	GLGGMEAKVKAALWA	succinylation	[1]
347	ITDIVEGKKVGTFFS	acetylation	[1, 2]
347	ITDIVEGKKVGTFFS	succinylation	[1]
348	TDIVEGKKVGTFFSE	acetylation	[3]
424	RLASPLLKRLSLSTS	acetylation	[1, 3, 4]
424	RLASPLLKRLSLSTS	succinylation	[1]
550	EDLCRLDKIIDLIIP	acetylation	[1, 3]
550	EDLCRLDKIIDLIIP	succinylation	[1]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine (By similarity). 

Sequence Annotation

 NP_BIND 266 267 ATP (By similarity).
 NP_BIND 305 311 ATP (By similarity).
 REGION 1 361 Glutamate 5-kinase.
 REGION 362 795 Gamma-glutamyl phosphate reductase.
 BINDING 117 117 Substrate (By similarity).
 BINDING 223 223 Substrate (By similarity).
 BINDING 246 246 Substrate; via amide nitrogen (By  

Keyword

 Alternative splicing; Amino-acid biosynthesis; ATP-binding; Complete proteome; Kinase; Membrane; Mitochondrion; Mitochondrion inner membrane; Multifunctional enzyme; NADP; Nucleotide-binding; Oxidoreductase; Proline biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 795 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004349; F:glutamate 5-kinase activity; IDA:MGI.
 GO:0004350; F:glutamate-5-semialdehyde dehydrogenase activity; ISS:UniProtKB.
 GO:0019240; P:citrulline biosynthetic process; ISS:UniProtKB.
 GO:0006536; P:glutamate metabolic process; ISS:UniProtKB.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006592; P:ornithine biosynthetic process; IEA:Compara.
 GO:0006561; P:proline biosynthetic process; ISS:UniProtKB. 

Interpro

 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom.
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR000965; G-glutamylP_reductase.
 IPR020593; G-glutamylP_reductase_CS.
 IPR001057; Glu/AcGlu_kinase.
 IPR019797; Glutamate_5-kinase_CS.
 IPR005766; P5_carboxy_syn. 

Pfam

 PF00696; AA_kinase
 PF00171; Aldedh 

SMART

  

PROSITE

 PS00902; GLUTAMATE_5_KINASE
 PS01223; PROA 

PRINTS

 PR00474; GLU5KINASE.