CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033464
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 N-lysine methyltransferase SETD8 
Protein Synonyms/Alias
  
Gene Name
 SETD8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
171GKTQQNRKLTDFYPVubiquitination[1]
184PVRRSSRKSKAELQSubiquitination[1]
186RRSSRKSKAELQSEEubiquitination[1, 2, 3, 4, 5]
195ELQSEERKRIDELIEubiquitination[2, 3]
205DELIESGKEEGMKIDubiquitination[2, 3]
217KIDLIDGKGRGVIATubiquitination[1, 2]
225GRGVIATKQFSRGDFubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 299 AA 
Protein Sequence
MRWLWLLLFP DSPVTLREVL PSQPWRLRGC SDLRGENVFT GQSKIYSYMS PNKCSGMRFP 60
LQEENSVTHH EVKCQGKPLA GIYRKREEKR NAGNAVRSAM KSEEQKIKDA RKGPLVPFPN 120
QKSEAAEPPK TPPSSCDSTN AAIAKQALKK PIKGKQAPRK KAQGKTQQNR KLTDFYPVRR 180
SSRKSKAELQ SEERKRIDEL IESGKEEGMK IDLIDGKGRG VIATKQFSRG DFVVEYHGDL 240
IEITDAKKRE ALYAQDPSTG CYMYYFQYLS KTYCVDATRE TNRLGRLINH SKCGNCQTK 299 
Gene Ontology
 GO:0018024; F:histone-lysine N-methyltransferase activity; IEA:InterPro.
 GO:0034968; P:histone lysine methylation; IEA:GOC. 
Interpro
 IPR016858; Hist_H4-K20_MeTrfase.
 IPR001214; SET_dom. 
Pfam
 PF00856; SET 
SMART
 SM00317; SET 
PROSITE
 PS51571; SAM_MT43_PR_SET
 PS50280; SET 
PRINTS