CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004763
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine synthase 2 
Protein Synonyms/Alias
 AdoMet synthase 2; Methionine adenosyltransferase 2; MAT 2 
Gene Name
 SAM2 
Gene Synonyms/Alias
 ETH2; YDR502C; D9719.8 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
5***MSKSKTFLFTSEacetylation[1]
42LEQDPFSKVACETAAubiquitination[2]
77QIVRDTIKKIGYDDSubiquitination[3, 4, 5]
78IVRDTIKKIGYDDSAubiquitination[3, 4, 5]
86IGYDDSAKGFDYKTCubiquitination[3, 4, 5]
213DLRTQLQKDIVEKVIacetylation[1]
213DLRTQLQKDIVEKVIubiquitination[2]
218LQKDIVEKVIPKDMLacetylation[1]
222IVEKVIPKDMLDENTacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [4] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [5] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. 
Sequence Annotation
 NP_BIND 120 125 ATP (Potential).
 METAL 20 20 Magnesium (By similarity).
 METAL 46 46 Potassium (By similarity).
 METAL 272 272 Potassium (By similarity).
 METAL 280 280 Magnesium (By similarity).
 BINDING 148 148 ATP (Potential).
 MOD_RES 2 2 N-acetylserine.
 CROSSLNK 77 77 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 78 78 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 86 86 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; ATP-binding; Cobalt; Complete proteome; Isopeptide bond; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 384 AA 
Protein Sequence
MSKSKTFLFT SESVGEGHPD KICDQVSDAI LDACLEQDPF SKVACETAAK TGMIMVFGEI 60
TTKARLDYQQ IVRDTIKKIG YDDSAKGFDY KTCNVLVAIE QQSPDIAQGL HYEKSLEDLG 120
AGDQGIMFGY ATDETPEGLP LTILLAHKLN MAMADARRDG SLPWLRPDTK TQVTVEYEDD 180
NGRWVPKRID TVVISAQHAD EISTADLRTQ LQKDIVEKVI PKDMLDENTK YFIQPSGRFV 240
IGGPQGDAGL TGRKIIVDAY GGASSVGGGA FSGKDYSKVD RSAAYAARWV AKSLVAAGLC 300
KRVQVQFSYA IGIAEPLSLH VDTYGTATKS DDEIIEIIKK NFDLRPGVLV KELDLARPIY 360
LPTASYGHFT NQEYSWEKPK KLEF 384 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004478; F:methionine adenosyltransferase activity; IDA:SGD.
 GO:0006555; P:methionine metabolic process; IMP:SGD.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IMP:SGD. 
Interpro
 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 
Pfam
 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 
SMART
  
PROSITE
 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 
PRINTS