CPLM-010887

Genbank Nucleotide ID

DNA-binding protein SATB1

Protein Synonyms/Alias

Special AT-rich sequence-binding protein 1

Homo sapiens (Human)

Position	Peptide	Type	References
136	KGLIQVGKWNPVPLS	acetylation	[1, 2]
744	TNTLFSVKLEEELSV	sumoylation	[3, 4]

[1] Phosphorylation of SATB1, a global gene regulator, acts as a molecular switch regulating its transcriptional activity in vivo.
Pavan Kumar P, Purbey PK, Sinha CK, Notani D, Limaye A, Jayani RS, Galande S.
Mol Cell. 2006 Apr 21;22(2):231-43. [PMID: 16630892]
[2] Acetylation-dependent interaction of SATB1 and CtBP1 mediates transcriptional repression by SATB1.
Purbey PK, Singh S, Notani D, Kumar PP, Limaye AS, Galande S.
Mol Cell Biol. 2009 Mar;29(5):1321-37. [PMID: 19103759]
[3] SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage.
Tan JA, Sun Y, Song J, Chen Y, Krontiris TG, Durrin LK.
J Biol Chem. 2008 Jun 27;283(26):18124-34. [PMID: 18408014]
[4] Phosphorylation-dependent interaction of SATB1 and PIAS1 directs SUMO-regulated caspase cleavage of SATB1.
Tan JA, Song J, Chen Y, Durrin LK.
Mol Cell Biol. 2010 Jun;30(11):2823-36. [PMID: 20351170]

Functional Description

Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1- binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.

DNA_BIND 361 448 CUT 1.
DNA_BIND 484 571 CUT 2.
DNA_BIND 645 704 Homeobox.
REGION 90 204 PDZ-like dimerization domain.
REGION 224 278 Nuclear matrix targeting sequence (NMTS).
REGION 400 410 Matrix attachment region (MAR) DNA-
MOTIF 20 40 Nuclear localization signal.
MOTIF 139 143 Protein interaction.
BINDING 390 390 Matrix attachment region (MAR) DNA.
BINDING 425 425 Matrix attachment region (MAR) DNA.
MOD_RES 136 136 N6-acetyllysine.
MOD_RES 185 185 Phosphoserine.
CROSSLNK 744 744 Glycyl lysine isopeptide (Lys-Gly)

3D-structure; Acetylation; Alternative splicing; Chromatin regulator; Chromosomal rearrangement; Complete proteome; DNA-binding; Homeobox; Host-virus interaction; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005720; C:nuclear heterochromatin; IEA:Compara.
GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
GO:0016605; C:PML body; IDA:UniProtKB.
GO:0003682; F:chromatin binding; IEA:Compara.
GO:0003690; F:double-stranded DNA binding; TAS:ProtInc.
GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
GO:0050798; P:activated T cell proliferation; IEA:Compara.
GO:0043367; P:CD4-positive, alpha-beta T cell differentiation; IEA:Compara.
GO:0043374; P:CD8-positive, alpha-beta T cell differentiation; IEA:Compara.
GO:0006921; P:cellular component disassembly involved in execution phase of apoptosis; TAS:Reactome.
GO:0006325; P:chromatin organization; TAS:ProtInc.
GO:0006338; P:chromatin remodeling; IEA:Compara.
GO:0008544; P:epidermis development; IEA:Compara.
GO:0016571; P:histone methylation; IEA:Compara.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:MGI.
GO:0060004; P:reflex; IEA:Compara.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR003350; Hmoeo_CUT.
IPR001356; Homeodomain.
IPR009057; Homeodomain-like.
IPR010982; Lambda_DNA-bd_dom.

PF02376; CUT
PF00046; Homeobox

PS51042; CUT
PS00027; HOMEOBOX_1
PS50071; HOMEOBOX_2