| Tag | Content |
|---|
| CPLM ID | CPLM-006395 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | General negative regulator of transcription subunit 4 |
Protein Synonyms/Alias | Modulator of transcription 2 |
Gene Name | MOT2 |
Gene Synonyms/Alias | CCL1; NOT4; SIG1; SSF1; YER068W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 97 | TLSPEELKMERAKLA | ubiquitination | [1] | | 168 | KYFGQYGKINKIVVN | ubiquitination | [1, 2] | | 171 | GQYGKINKIVVNRKT | ubiquitination | [1] | | 205 | VYITFGSKDDAARCI | ubiquitination | [1] | | 226 | YMDGRLIKAAYGTTK | ubiquitination | [1] | | 264 | EEADSFNKRELHNKQ | ubiquitination | [1] | | 270 | NKRELHNKQQAQQQS | ubiquitination | [1, 2] | | 417 | SLAAEEYKDPYDALG | ubiquitination | [2] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] |
Functional Description | Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC- type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. |
Sequence Annotation | DOMAIN 137 228 RRM.
ZN_FING 33 78 RING-type.
ZN_FING 229 256 C3H1-type.
MOD_RES 310 310 Phosphothreonine.
MOD_RES 312 312 Phosphoserine.
MOD_RES 326 326 Phosphothreonine.
MOD_RES 360 360 Phosphoserine. |
Keyword | Activator; Coiled coil; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-binding; Transcription; Transcription regulation; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 587 AA |
Protein Sequence | MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC PCGYQICQFC 60
YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER AKLARKEKER KHREKERKEN 120
EYTNRKHLSG TRVIQKNLVY VVGINPPVPY EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH 180
SNNTTSEHYH HHSPGYGVYI TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR 240
GLPCPNPNCM FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT 300
NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS ATPVTSINLS 360
KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS HSHGSKKKQS LAAEEYKDPY 420
DALGNAVDFL DARLHSLSNY QKRPISIKSN IIDEETYKKY PSLFSWDKIE ASKKSDNTLA 480
NKLVEILAIK PIDYTASVVQ FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN 540
VSTPPPGIFG PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN 587 |
Gene Ontology | GO:0030015; C:CCR4-NOT core complex; IPI:SGD. GO:0005737; C:cytoplasm; IDA:SGD. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0000166; F:nucleotide binding; IEA:InterPro. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0043565; F:sequence-specific DNA binding; IDA:SGD. GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0031087; P:deadenylation-independent decapping of nuclear-transcribed mRNA; IMP:SGD. GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD. GO:0000209; P:protein polyubiquitination; IDA:SGD. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |