CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031725
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 USP19 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
650DLNRIQNKPYTETVDubiquitination[1, 2, 3]
691DLFQGQYKSKLVCPVubiquitination[1, 3, 4]
693FQGQYKSKLVCPVCAubiquitination[2, 3]
775LRLAEVIKNRFHRVFubiquitination[2, 3]
863YCNQLCQKTHWPDHKubiquitination[2]
1160RSFIWRDKINDLVEFubiquitination[1, 2, 3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Metal-binding; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1358 AA 
Protein Sequence
MSGGASATGP RRGPPGLEDT TSKKKQKDRA NQESKDGDPR KASTPQEEQT KEELLLDWRQ 60
SAEEVIVKLR VGVGPLQLED VDAAFTDTDC VVRFAGGQQW GGVFYAEIKS SCAKVQTRKG 120
SLLHLTLPKK VPMLTWPSLL KPLGTQELVP GLRCQENGQE LSPIALEPGP EPHRAKQEAR 180
NQKRAQGRGE VGAGAGPGAQ AGPSAKRAVH LCRGPEGDGS RDDPGPRGDA PPFVADPATQ 240
VEADEQLCIP PLNSQTCLLG SEENLAPLAG EKAVPPGNDP VSPAMVRSRN PGKDDCAKEE 300
MAVAADAATL VDEPESMVNL AFVKNDSYEK GPDSVVVHVY VKEICRDTSR VLFREQDFTL 360
IFQTRDGNFL RLHPGCGPHT TFRWQVKLRN LIEPEQCTFC FTASRIDICL RKRQSQRWGG 420
LEAPAARGAV GGAKVAVPTG PTPLDSTPPG GAPHPLTGQE EARAVEKDKS KARSEDTGLD 480
SVATRTPMEH VTPKPETHLA SPKPTCMVPP MPHSPVSGDS VEEEEEEEKK VCLPGFTGLV 540
NLGNTCFMNS VIQSLSNTRE LRDFFHDRSF EAEINYNNPL GTGGRLAIGF AVLLRALWKG 600
THHAFQPSKL KAIVASKASQ FTGYAQHDAQ EFMAFLLDGL HEDLNRIQNK PYTETVDSDG 660
RPDEVVAEEA WQRHKMRNDS FIVDLFQGQY KSKLVCPVCA KVSITFDPFL YLPVPLPQKQ 720
KVLPVFYFAR EPHSKPIKFL VSVSKENSTA SEVLDSLSQS VHVKPENLRL AEVIKNRFHR 780
VFLPSHSLDT VSPSDTLLCF ELLSSELAKE RVVVLEVQQR PQVPSVPISK CAACQRKQQS 840
EDEKLKRCTR CYRVGYCNQL CQKTHWPDHK GLCRPENIGY PFLVSVPASR LTYARLAQLL 900
EGYARYSVSV FQPPFQPGRM ALESQSPGCT TLLSTGSLEA GDSERDPIQP PELQLVTPMA 960
EGDTGLPRVW AAPDRGPVPS TSGISSEMLA SGPIEVGSLP AGERVSRPEA AVPGYQHPSE 1020
AMNAHTPQFF IYKIDSSNRE QRLEDKGDTP LELGDDCSLA LVWRNNERLQ EFVLVASKEL 1080
ECAEDPGSAG EAARAGHFTL DQCLNLFTRP EVLAPEEAWY CPQCKQHREA SKQLLLWRLP 1140
NVLIVQLKRF SFRSFIWRDK INDLVEFPVR NLDLSKFCIG QKEEQLPSYD LYAVINHYGG 1200
MIGGHYTACA RLPNDRSSQR SDVGWRLFDD STVTTVDESQ VVTRYAYVLF YRRRNSPVER 1260
PPRAGHSEHH PDLGPAAEAA ASQASRIWQE LEAEEEPVPE GSGPLGPWGP QDWVGPLPRG 1320
PTTPDEGCLR YFVLGTVAAL VALVLNVFYP LVSQSRWR 1358 
Gene Ontology
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR007052; CS-like_domain.
 IPR017447; CS_domain.
 IPR015054; DUF1872.
 IPR008978; HSP20-like_chaperone.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR002893; Znf_MYND. 
Pfam
 PF04969; CS
 PF08959; DUF1872
 PF00443; UCH
 PF01753; zf-MYND 
SMART
  
PROSITE
 PS51203; CS
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3
 PS01360; ZF_MYND_1
 PS50865; ZF_MYND_2 
PRINTS