CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015393
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Monofunctional C1-tetrahydrofolate synthase, mitochondrial 
Protein Synonyms/Alias
 Formyltetrahydrofolate synthetase 
Gene Name
 MTHFD1L 
Gene Synonyms/Alias
 FTHFSDC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
174NKVLNALKPEKDVDGacetylation[1]
189VTDINLGKLVRGDAHacetylation[1]
516HENTQTDKALYNRLVacetylation[2, 3]
516HENTQTDKALYNRLVubiquitination[4]
596IGQGNTEKGHYRQAQacetylation[3]
727MEKFFNIKCRASGLVubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2 (By similarity). 
Sequence Annotation
 NP_BIND 423 430 ATP (By similarity).
 REGION 31 348 Methylenetetrahydrofolate dehydrogenase
 REGION 349 978 Formyltetrahydrofolate synthetase.
 MOD_RES 189 189 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Mitochondrion; Nucleotide-binding; One-carbon metabolism; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 978 AA 
Protein Sequence
MGTRLPLVLR QLRRPPQPPG PPRRLRVPCR ASSGGGGGGG GGREGLLGQR RPQDGQARSS 60
CSPGGRTPAA RDSIVREVIQ NSKEVLSLLQ EKNPAFKPVL AIIQAGDDNL MQEINQNLAE 120
EAGLNITHIC LPPDSSEAEI IDEILKINED TRVHGLALQI SENLFSNKVL NALKPEKDVD 180
GVTDINLGKL VRGDAHECFV SPVAKAVIEL LEKSGVNLDG KKILVVGAHG SLEAALQCLF 240
QRKGSMTMSI QWKTRQLQSK LHEADIVVLG SPKPEEIPLT WIQPGTTVLN CSHDFLSGKV 300
GCGSPRIHFG GLIEEDDVIL LAAALRIQNM VSSGRRWLRE QQHRRWRLHC LKLQPLSPVP 360
SDIEISRGQT PKAVDVLAKE IGLLADEIEI YGKSKAKVRL SVLERLKDQA DGKYVLVAGI 420
TPTPLGEGKS TVTIGLVQAL TAHLNVNSFA CLRQPSQGPT FGVKGGAAGG GYAQVIPMEE 480
FNLHLTGDIH AITAANNLLA AAIDTRILHE NTQTDKALYN RLVPLVNGVR EFSEIQLARL 540
KKLGINKTDP STLTEEEVSK FARLDIDPST ITWQRVLDTN DRFLRKITIG QGNTEKGHYR 600
QAQFDIAVAS EIMAVLALTD SLADMKARLG RMVVASDKSG QPVTADDLGV TGALTVLMKD 660
AIKPNLMQTL EGTPVFVHAG PFANIAHGNS SVLADKIALK LVGEEGFVVT EAGFGADIGM 720
EKFFNIKCRA SGLVPNVVVL VATVRALKMH GGGPSVTAGV PLKKEYTEEN IQLVADGCCN 780
LQKQIQITQL FGVPVVVALN VFKTDTRAEI DLVCELAKRA GAFDAVPCYH WSVGGKGSVD 840
LARAVREAAS KRSRFQFLYD VQVPIVDKIR TIAQAVYGAK DIELSPEAQA KIDRYTQQGF 900
GNLPICMAKT HLSLSHQPDK KGVPRDFILP ISDVRASIGA GFIYPLVGTM STMPGLPTRP 960
CFYDIDLDTE TEQVKGLF 978 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IDA:BHF-UCL.
 GO:0004329; F:formate-tetrahydrofolate ligase activity; IDA:BHF-UCL.
 GO:0042803; F:protein homodimerization activity; IDA:BHF-UCL.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0015942; P:formate metabolic process; IDA:BHF-UCL.
 GO:0006730; P:one-carbon metabolic process; IBA:RefGenome.
 GO:0055114; P:oxidation-reduction process; IEA:InterPro.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
 GO:0046653; P:tetrahydrofolate metabolic process; IDA:BHF-UCL. 
Interpro
 IPR000559; Formate_THF_ligase.
 IPR020628; Formate_THF_ligase_CS.
 IPR016040; NAD(P)-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 
Pfam
 PF01268; FTHFS
 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 
SMART
  
PROSITE
 PS00721; FTHFS_1
 PS00722; FTHFS_2 
PRINTS
 PR00085; THFDHDRGNASE.