CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002948
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate--cysteine ligase 
Protein Synonyms/Alias
 Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase 
Gene Name
 gshA 
Gene Synonyms/Alias
 gsh-I; b2688; JW2663 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
220PTSLPFEKTECGMYYacetylation[1]
276TPSEEYAKIGIEKDGacetylation[1]
281YAKIGIEKDGKRLQIacetylation[1]
465TGIGGTGKAFAEAYRacetylation[1]
516PFAVWLEKHA*****acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
  
Keyword
 3D-structure; ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 518 AA 
Protein Sequence
MIPDVSQALA WLEKHPQALK GIQRGLERET LRVNADGTLA TTGHPEALGS ALTHKWITTD 60
FAEALLEFIT PVDGDIEHML TFMRDLHRYT ARNMGDERMW PLSMPCYIAE GQDIELAQYG 120
TSNTGRFKTL YREGLKNRYG ALMQTISGVH YNFSLPMAFW QAKCGDISGA DAKEKISAGY 180
FRVIRNYYRF GWVIPYLFGA SPAICSSFLQ GKPTSLPFEK TECGMYYLPY ATSLRLSDLG 240
YTNKSQSNLG ITFNDLYEYV AGLKQAIKTP SEEYAKIGIE KDGKRLQINS NVLQIENELY 300
APIRPKRVTR SGESPSDALL RGGIEYIEVR SLDINPFSPI GVDEQQVRFL DLFMVWCALA 360
DAPEMSSSEL ACTRVNWNRV ILEGRKPGLT LGIGCETAQF PLPQVGKDLF RDLKRVAQTL 420
DSINGGEAYQ KVCDELVACF DNPDLTFSAR ILRSMIDTGI GGTGKAFAEA YRNLLREEPL 480
EILREEDFVA EREASERRQQ EMEAADTEPF AVWLEKHA 518 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004357; F:glutamate-cysteine ligase activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IDA:EcoCyc.
 GO:0071243; P:cellular response to arsenic-containing substance; IMP:EcoCyc.
 GO:0071288; P:cellular response to mercury ion; IMP:EcoCyc.
 GO:0006750; P:glutathione biosynthetic process; IMP:EcoCyc.
 GO:0006972; P:hyperosmotic response; IMP:EcoCyc. 
Interpro
 IPR007370; Glu_cys_ligase.
 IPR006334; Glut_cys_ligase. 
Pfam
 PF04262; Glu_cys_ligase 
SMART
  
PROSITE
  
PRINTS