CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018318
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trafficking protein particle complex subunit 12 
Protein Synonyms/Alias
 Tetratricopeptide repeat protein 15; TPR repeat protein 15; TTC-15 
Gene Name
 TRAPPC12 
Gene Synonyms/Alias
 TTC15; CGI-87 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
181GFEPQMVKSPSFGGAubiquitination[1]
335RGAVFVDKENLTMPGubiquitination[1]
354NIQGDAVKDLMLRFLubiquitination[1, 2, 3, 4, 5]
364MLRFLGEKAAAKRQVubiquitination[1]
385EQSFVGLKQLISCRNubiquitination[1]
510KVKTVCSKILANLEQubiquitination[1]
597SLQIGDIKTAEKYFQubiquitination[1, 6]
601GDIKTAEKYFQDVEKubiquitination[1, 2, 5]
608KYFQDVEKVTQKLDGubiquitination[1]
612DVEKVTQKLDGLQGKubiquitination[1]
712SSRSMQKKQALLEAVubiquitination[1]
722LLEAVAGKEGDSFNTubiquitination[1]
733SFNTQCLKLA*****ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May be involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage. 
Sequence Annotation
 REPEAT 545 578 TPR 1.
 REPEAT 580 613 TPR 2.
 REPEAT 620 653 TPR 3.
 REPEAT 654 687 TPR 4.
 MOD_RES 109 109 Phosphoserine (By similarity).
 MOD_RES 184 184 Phosphoserine.  
Keyword
 Complete proteome; ER-Golgi transport; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 735 AA 
Protein Sequence
MEDAGGGEET PAPEAPHPPQ LAPPEEQGLL FQEETIDLGG DEFGSEENET ASEGSSPLAD 60
KLNEHMMESV LISDSPNSEG DAGDLGRVRD EAEPGGEGDP GPEPAGTPSP SGEADGDCAP 120
EDAAPSSGGA PRQDAAREVP GSEAARPEQE PPVAEPVPVC TIFSQRAPPA SGDGFEPQMV 180
KSPSFGGASE ASARTPPQVV QPSPSLSTFF GDTAASHSLA SDFFDSFTTS AFISVSNPGA 240
GSPAPASPPP LAVPGTEGRP EPVAMRGPQA AAPPASPEPF AHIQAVFAGS DDPFATALSM 300
SEMDRRNDAW LPGEATRGVL RAVATQQRGA VFVDKENLTM PGLRFDNIQG DAVKDLMLRF 360
LGEKAAAKRQ VLNADSVEQS FVGLKQLISC RNWRAAVDLC GRLLTAHGQG YGKSGLLTSH 420
TTDSLQLWFV RLALLVKLGL FQNAEMEFEP FGNLDQPDLY YEYYPHVYPG RRGSMVPFSM 480
RILHAELQQY LGNPQESLDR LHKVKTVCSK ILANLEQGLA EDGGMSSVTQ EGRQASIRLW 540
RSRLGRVMYS MANCLLLMKD YVLAVEAYHS VIKYYPEQEP QLLSGIGRIS LQIGDIKTAE 600
KYFQDVEKVT QKLDGLQGKI MVLMNSAFLH LGQNNFAEAH RFFTEILRMD PRNAVANNNA 660
AVCLLYLGKL KDSLRQLEAM VQQDPRHYLH ESVLFNLTTM YELESSRSMQ KKQALLEAVA 720
GKEGDSFNTQ CLKLA 735 
Gene Ontology
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS