| Tag | Content |
|---|
| CPLM ID | CPLM-004967 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ribonuclease R |
Protein Synonyms/Alias | RNase R; Protein VacB |
Gene Name | rnr |
Gene Synonyms/Alias | vacB; yjeC; b4179; JW5741 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 13 | FQEREAEKYANPIPS | acetylation | [1] | | 30 | FILEHLTKREKPASR | acetylation | [1] | | 86 | PERLDLVKGTVIGHR | acetylation | [1] | | 105 | FLRVEGRKDDLYLSS | acetylation | [1] | | 249 | EQQVAGLKEEVPEEA | acetylation | [1] | | 287 | DDAVYCEKKRGGGWR | acetylation | [1] | | 369 | KGRLTGYKFYEAVMS | acetylation | [1] | | 385 | HARLTYTKVWHILQG | acetylation | [1] | | 405 | EQYAPLVKHLEELHN | acetylation | [1] | | 415 | EELHNLYKVLDKARE | acetylation | [1] | | 488 | ALFRIHDKPSTEAIT | acetylation | [1] | | 513 | LELPGGNKPEPRDYA | acetylation | [1] | | 544 | TMLLRSMKQAIYDPE | acetylation | [1, 2] | | 719 | AVNMDERKIDFSLIS | acetylation | [1] | | 773 | DSAFRGEKKTKPKAA | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli. Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y. Mol Cell Proteomics. 2009 Feb;8(2):215-25. [ PMID: 18723842] |
Functional Description | 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (rRNAs, tRNAs and SsrA/tmRNA). In stationary phase, involved in the post-transcriptional regulation of ompA mRNA stability. Shortens RNA processively to di- and trinucleotides. In vitro, exhibits helicase activity, which is independent of its RNase activity. Required for the expression of virulence genes in enteroinvasive strains of E.coli. |
Sequence Annotation | DOMAIN 644 725 S1 motif.
MOD_RES 544 544 N6-acetyllysine. |
Keyword | Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Exonuclease; Hydrolase; Nuclease; Reference proteome; RNA-binding; Stress response; Virulence. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 813 AA |
Protein Sequence | MSQDPFQERE AEKYANPIPS REFILEHLTK REKPASRDEL AVELHIEGEE QLEGLRRRLR 60
AMERDGQLVF TRRQCYALPE RLDLVKGTVI GHRDGYGFLR VEGRKDDLYL SSEQMKTCIH 120
GDQVLAQPLG ADRKGRREAR IVRVLVPKTS QIVGRYFTEA GVGFVVPDDS RLSFDILIPP 180
DQIMGARMGF VVVVELTQRP TRRTKAVGKI VEVLGDNMGT GMAVDIALRT HEIPYIWPQA 240
VEQQVAGLKE EVPEEAKAGR VDLRDLPLVT IDGEDARDFD DAVYCEKKRG GGWRLWVAIA 300
DVSYYVRPST PLDREARNRG TSVYFPSQVI PMLPEVLSNG LCSLNPQVDR LCMVCEMTVS 360
SKGRLTGYKF YEAVMSSHAR LTYTKVWHIL QGDQDLREQY APLVKHLEEL HNLYKVLDKA 420
REERGGISFE SEEAKFIFNA ERRIERIEQT QRNDAHKLIE ECMILANISA ARFVEKAKEP 480
ALFRIHDKPS TEAITSFRSV LAELGLELPG GNKPEPRDYA ELLESVADRP DAEMLQTMLL 540
RSMKQAIYDP ENRGHFGLAL QSYAHFTSPI RRYPDLTLHR AIKYLLAKEQ GHQGNTTETG 600
GYHYSMEEML QLGQHCSMAE RRADEATRDV ADWLKCDFML DQVGNVFKGV ISSVTGFGFF 660
VRLDDLFIDG LVHVSSLDND YYRFDQVGQR LMGESSGQTY RLGDRVEVRV EAVNMDERKI 720
DFSLISSERA PRNVGKTARE KAKKGDAGKK GGKRRQVGKK VNFEPDSAFR GEKKTKPKAA 780
KKDARKAKKP SAKTQKIAAA TKAKRAAKKK VAE 813 |
Gene Ontology | |
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Pfam | |
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