UniProt Accession

 THOP1_HUMAN; P52888; Q9UCB3 

Genbank Protein ID

 Z50115; U29366; AC006538; BC000135; BC002391 

Genbank Nucleotide ID

 CAA90477.1; AAA82607.1; AAD13118.1; AAH00135.1; AAH02391.1 

Protein Name

 Thimet oligopeptidase 

Protein Synonyms/Alias

 Endopeptidase 24.15; MP78 

Gene Name

 THOP1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
92	PQHVSPSKDIRTAST	ubiquitination	[1]
103	TASTEADKKLSEFDV	ubiquitination	[1]
104	ASTEADKKLSEFDVE	ubiquitination	[1]
128	RIVWLQEKVQKDSLR	ubiquitination	[1]
131	WLQEKVQKDSLRPEA	ubiquitination	[1]
170	NIKRIKKKLSLLCID	ubiquitination	[1]
216	KMEDGKLKVTLKYPH	ubiquitination	[1]
220	GKLKVTLKYPHYFPL	ubiquitination	[1, 2, 3]
229	PHYFPLLKKCHVPET	acetylation	[4]
230	HYFPLLKKCHVPETR	ubiquitination	[1]
239	HVPETRRKVEEAFNC	ubiquitination	[1]
257	EENCAILKELVTLRA	acetylation	[4]
257	EENCAILKELVTLRA	ubiquitination	[1]
303	DELAQKLKPLGEQER	ubiquitination	[1]
317	RAVILELKRAECERR	ubiquitination	[1, 5]
421	DLYPREGKYGHAACF	ubiquitination	[1]
538	VPRELLEKLIESRQA	acetylation	[4]
538	VPRELLEKLIESRQA	ubiquitination	[1, 2, 3, 5]
559	LRQIVLAKVDQALHT	ubiquitination	[1, 5]
628	DMFHTRFKQEGVLNS	ubiquitination	[1]
636	QEGVLNSKVGMDYRS	ubiquitination	[1]
667	RFLGRDPKQDAFLLS	ubiquitination	[1]
675	QDAFLLSKGLQVGGC	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments. 

Sequence Annotation

 ACT_SITE 474 474
 METAL 473 473 Zinc; catalytic.
 METAL 477 477 Zinc; catalytic.
 METAL 480 480 Zinc; catalytic.
 MOD_RES 257 257 N6-acetyllysine.
 MOD_RES 278 278 Phosphotyrosine (By similarity).
 MOD_RES 538 538 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 689 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; TAS:ProtInc.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0007243; P:intracellular protein kinase cascade; IEA:Compara.
 GO:0006518; P:peptide metabolic process; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR024079; MetalloPept_cat_dom.
 IPR024077; Neurolysin/TOP_dom2.
 IPR024080; Neurolysin/TOP_N.
 IPR001567; Pept_M3A_M3B. 

Pfam

 PF01432; Peptidase_M3 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS