CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002238
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Non-histone chromosomal protein HMG-17 
Protein Synonyms/Alias
 High mobility group nucleosome-binding domain-containing protein 2 
Gene Name
 HMGN2 
Gene Synonyms/Alias
 HMG17 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
3*****MPKRKAEGDAacetylation[1]
31RSARLSAKPAPPKPEacetylation[2, 3, 4, 5]
31RSARLSAKPAPPKPEubiquitination[5, 6, 7]
36SAKPAPPKPEPKPKKacetylation[2, 3, 4, 5]
40APPKPEPKPKKAPAKacetylation[4, 5]
59VPKGKKGKADAGKEGacetylation[5, 8]
64KGKADAGKEGNNPAEacetylation[4, 5, 8]
64KGKADAGKEGNNPAEubiquitination[5, 6, 9]
76PAENGDAKTDQAQKAacetylation[3, 5]
76PAENGDAKTDQAQKAubiquitination[5, 6, 7, 9]
82AKTDQAQKAEGAGDAacetylation[2, 3, 4, 5]
82AKTDQAQKAEGAGDAubiquitination[7, 9, 10, 11]
Reference
 [1] Specific acetylation of chromosomal protein HMG-17 by PCAF alters its interaction with nucleosomes.
 Herrera JE, Sakaguchi K, Bergel M, Trieschmann L, Nakatani Y, Bustin M.
 Mol Cell Biol. 1999 May;19(5):3466-73. [PMID: 10207070]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [10] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [11] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation (By similarity). 
Sequence Annotation
 MOD_RES 25 25 Phosphoserine.
 MOD_RES 29 29 Phosphoserine.
 MOD_RES 82 82 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 90 AA 
Protein Sequence
MPKRKAEGDA KGDKAKVKDE PQRRSARLSA KPAPPKPEPK PKKAPAKKGE KVPKGKKGKA 60
DAGKEGNNPA ENGDAKTDQA QKAEGAGDAK 90 
Gene Ontology
 GO:0000785; C:chromatin; IEA:InterPro.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0031492; F:nucleosomal DNA binding; IEA:InterPro.
 GO:0006325; P:chromatin organization; NAS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB. 
Interpro
 IPR000079; HMGN_fam. 
Pfam
 PF01101; HMG14_17 
SMART
 SM00527; HMG17 
PROSITE
 PS00355; HMG14_17 
PRINTS
 PR00925; NONHISHMG17.