UniProt Accession

 GHRB_ECOLI; P37666; Q2M7L4 

Genbank Protein ID

 U00039; U00096; AP009048 

Genbank Nucleotide ID

 AAB18530.1; AAC76577.2; BAE77742.1 

Protein Name

 Glyoxylate/hydroxypyruvate reductase B 

Protein Synonyms/Alias

 2-ketoaldonate reductase; 2-ketogluconate reductase; 2KR 

Gene Name

 ghrB 

Gene Synonyms/Alias

 tkrA; yiaE; b3553; JW5656 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
9	KPSVILYKALPDDLL	acetylation	[1]
181	YNARRHHKEAEERFN	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Can also reduce 2,5-diketo-D-gluconate (25DKG) to 5-keto-D- gluconate (5KDG), 2-keto-D-gluconate (2KDG) to D-gluconate, and 2- keto-L-gulonate (2KLG) to L-idonate (IA), but it is not its physiological function. Inactive towards 2-oxoglutarate, oxaloacetate, pyruvate, 5-keto-D-gluconate, D-fructose and L- sorbose. Activity with NAD is very low. 

Sequence Annotation

 ACT_SITE 237 237 By similarity.
 ACT_SITE 266 266 By similarity.
 ACT_SITE 285 285 Proton donor (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Gluconate utilization; NAD; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 324 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:HAMAP.
 GO:0008873; F:gluconate 2-dehydrogenase activity; IDA:EcoCyc.
 GO:0030267; F:glyoxylate reductase (NADP) activity; IDA:EcoCyc.
 GO:0016618; F:hydroxypyruvate reductase activity; IEA:HAMAP.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
 GO:0046181; P:ketogluconate catabolic process; IMP:EcoCyc. 

Interpro

 IPR006139; D-isomer_2_OHA_DH_cat_dom.
 IPR006140; D-isomer_2_OHA_DH_NAD-bd.
 IPR023756; Glyo/OHPyrv_Rdtase_B.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00389; 2-Hacid_dh
 PF02826; 2-Hacid_dh_C 

SMART

  

PROSITE

 PS00065; D_2_HYDROXYACID_DH_1
 PS00670; D_2_HYDROXYACID_DH_2
 PS00671; D_2_HYDROXYACID_DH_3 

PRINTS