CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001782
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aspartate aminotransferase 
Protein Synonyms/Alias
 AspAT; Transaminase A 
Gene Name
 aspC 
Gene Synonyms/Alias
 b0928; JW0911 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
28RADERPGKINLGIGVacetylation[1]
37NLGIGVYKDETGKTPacetylation[1, 2]
42VYKDETGKTPVLTSVacetylation[1]
51PVLTSVKKAEQYLLEacetylation[1, 2]
63LLENETTKNYLGIDGacetylation[1]
85TQELLFGKGSALINDacetylation[1]
93GSALINDKRARTAQTacetylation[1]
116VAADFLAKNTSVKRVacetylation[1]
134NPSWPNHKSVFNSAGacetylation[1]
276DRAFSQMKAAIRANYacetylation[1, 2]
332FVNTLQEKGANRDFSacetylation[1]
355FSFSGLTKEQVLRLRacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
  
Sequence Annotation
 BINDING 34 34 Aspartate; via amide nitrogen.
 BINDING 130 130 Aspartate.
 BINDING 183 183 Aspartate.
 BINDING 374 374 Aspartate.
 MOD_RES 246 246 N6-(pyridoxal phosphate)lysine.  
Keyword
 3D-structure; Aminotransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Pyridoxal phosphate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 396 AA 
Protein Sequence
MFENITAAPA DPILGLADLF RADERPGKIN LGIGVYKDET GKTPVLTSVK KAEQYLLENE 60
TTKNYLGIDG IPEFGRCTQE LLFGKGSALI NDKRARTAQT PGGTGALRVA ADFLAKNTSV 120
KRVWVSNPSW PNHKSVFNSA GLEVREYAYY DAENHTLDFD ALINSLNEAQ AGDVVLFHGC 180
CHNPTGIDPT LEQWQTLAQL SVEKGWLPLF DFAYQGFARG LEEDAEGLRA FAAMHKELIV 240
ASSYSKNFGL YNERVGACTL VAADSETVDR AFSQMKAAIR ANYSNPPAHG ASVVATILSN 300
DALRAIWEQE LTDMRQRIQR MRQLFVNTLQ EKGANRDFSF IIKQNGMFSF SGLTKEQVLR 360
LREEFGVYAV ASGRVNVAGM TPDNMAPLCE AIVAVL 396 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; IMP:EcoliWiki.
 GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC.
 GO:0004838; F:L-tyrosine:2-oxoglutarate aminotransferase activity; IMP:EcoliWiki.
 GO:0030170; F:pyridoxal phosphate binding; IDA:EcoliWiki.
 GO:0033585; P:L-phenylalanine biosynthetic process from chorismate via phenylpyruvate; IMP:EcoCyc. 
Interpro
 IPR004839; Aminotransferase_I/II.
 IPR000796; Asp_trans.
 IPR004838; NHTrfase_class1_PyrdxlP-BS.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1. 
Pfam
 PF00155; Aminotran_1_2 
SMART
  
PROSITE
 PS00105; AA_TRANSFER_CLASS_1 
PRINTS
 PR00799; TRANSAMINASE.