CPLM-003838

Genbank Nucleotide ID

S-formylglutathione hydrolase

Protein Synonyms/Alias

FGH; Esterase D; Methylumbelliferyl-acetate deacetylase

Homo sapiens (Human)

Position	Peptide	Type	References
4	****MALKQISSNKC	acetylation	[1]
4	****MALKQISSNKC	ubiquitination	[1, 2, 3]
10	LKQISSNKCFGGLQK	acetylation	[1]
10	LKQISSNKCFGGLQK	ubiquitination	[1, 2, 3]
17	KCFGGLQKVFEHDSV	ubiquitination	[1, 2, 3]
29	DSVELNCKMKFAVYL	ubiquitination	[1, 3]
31	VELNCKMKFAVYLPP	ubiquitination	[4, 5]
39	FAVYLPPKAETGKCP	acetylation	[6]
167	LKNPGKYKSVSAFAP	ubiquitination	[3, 4, 5, 7]
185	PVLCPWGKKAFSGYL	ubiquitination	[2, 3]
186	VLCPWGKKAFSGYLG	ubiquitination	[3]
198	YLGTDQSKWKAYDAT	ubiquitination	[3, 4, 5, 7]
200	GTDQSKWKAYDATHL	acetylation	[6]
200	GTDQSKWKAYDATHL	ubiquitination	[2, 3, 4, 5]
209	YDATHLVKSYPGSQL	ubiquitination	[4, 5]
247	IAACTEKKIPVVFRL	ubiquitination	[3]
278	DHIRHHAKYLNA***	acetylation	[6]

[1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Serine hydrolase involved in the detoxification of formaldehyde.

ACT_SITE 149 149 Charge relay system.
ACT_SITE 226 226 Charge relay system.
ACT_SITE 260 260 Charge relay system.
MOD_RES 200 200 N6-acetyllysine.

3D-structure; Acetylation; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Hydrolase; Polymorphism; Reference proteome; Serine esterase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016023; C:cytoplasmic membrane-bounded vesicle; NAS:UniProtKB.
GO:0005794; C:Golgi apparatus; IDA:HPA.
GO:0005634; C:nucleus; IDA:HPA.
GO:0004091; F:carboxylesterase activity; NAS:UniProtKB.
GO:0047374; F:methylumbelliferyl-acetate deacetylase activity; IEA:EC.
GO:0018738; F:S-formylglutathione hydrolase activity; IEA:EC.
GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.

IPR000801; Esterase_put.
IPR014186; S-formylglutathione_hydrol.